Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA.
Contains 1 B30.2/SPRY domain. Contains 1 SAP domain.
Extensively phosphorylated. Arg-733 and Arg-739 are dimethylated, probably to asymmetric dimethylarginine.
Nucleus. Cytoplasm. Cell surface. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Also found associated with the cell surface.
Heterogeneous nuclear ribonucleoprotein U antibody
hnRNP U antibody
hnRNP U protein antibody
hnRNPU protein antibody
p120 nuclear protein antibody
SAF A antibody
Scaffold attachment factor A antibody
Western blot - Anti-hnRNP U antibody [EPR12278(2)(B)] (ab180952)
All lanes : Anti-hnRNP U antibody [EPR12278(2)(B)] (ab180952) at 1/20000 dilution
Lane 1 : K562 cell lysate with NFDM/TBST Lane 2 : Jurkat cell lysate with NFDM/TBST Lane 3 : 293 cell lysate with NFDM/TBST
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
Secondary All lanes : Peroxidase conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 91 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-hnRNP U antibody [EPR12278(2)(B)] (ab180952)
Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney labeling hnRNP U using ab180952 at a 1/100 dilution, with Hematoxylin counter staining. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.
Immunocytochemistry/ Immunofluorescence - Anti-hnRNP U antibody [EPR12278(2)(B)] (ab180952)
Immunofluorescence analysis of -20℃ acetone-fixed Jurkat cells labeling hnRNP U using ab180952 at a 1/100 dilution (green) with Dapi counter staining (blue). Goat anti rabbit IgG (Dylight 488), at a dilution of 1/200, was used as a secondary antibody.
Flow Cytometry - Anti-hnRNP U antibody [EPR12278(2)(B)] (ab180952)
Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling hnRNP U using ab180952 at a 1/100 dilution, or Rabbit monoclonal IgG as an isotype control.