Recombinant
RabMAb

Anti-hnRNP U antibody [EPR12278(2)(B)] (ab180952)

Overview

  • Product name
    Anti-hnRNP U antibody [EPR12278(2)(B)]
    See all hnRNP U primary antibodies
  • Description
    Rabbit monoclonal [EPR12278(2)(B)] to hnRNP U
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human hnRNP U aa 50-150. The exact sequence is proprietary.
    Database link: Q00839

  • Positive control
    • K562, Jurkat and 293 cell lines; Human clear cell carcinoma of kidney tissue.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12278(2)(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab180952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
Flow Cyt 1/10.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100.
WB 1/10000 - 1/50000. Predicted molecular weight: 91 kDa.

Target

  • Function
    Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA.
  • Sequence similarities
    Contains 1 B30.2/SPRY domain.
    Contains 1 SAP domain.
  • Post-translational
    modifications
    Extensively phosphorylated.
    Arg-733 and Arg-739 are dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization
    Nucleus. Cytoplasm. Cell surface. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Also found associated with the cell surface.
  • Information by UniProt
  • Database links
  • Alternative names
    • Heterogeneous nuclear ribonucleoprotein U antibody
    • hnRNP U antibody
    • hnRNP U protein antibody
    • HNRNPU antibody
    • hnRNPU protein antibody
    • HNRPU antibody
    • HNRPU_HUMAN antibody
    • p120 antibody
    • p120 nuclear protein antibody
    • pp120 antibody
    • SAF A antibody
    • SAF-A antibody
    • SAFA antibody
    • Scaffold attachment factor A antibody
    • U21.1 antibody
    see all

Images

  • All lanes : Anti-hnRNP U antibody [EPR12278(2)(B)] (ab180952) at 1/20000 dilution

    Lane 1 : K562 cell lysate with NFDM/TBST
    Lane 2 : Jurkat cell lysate with NFDM/TBST
    Lane 3 : 293 cell lysate with NFDM/TBST

    Lysates/proteins at 20 µg per lane.

    Blocking peptides at 5 % per lane.

    Secondary
    All lanes : Peroxidase conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 91 kDa

  • Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney labeling hnRNP U using ab180952 at a 1/100 dilution, with Hematoxylin counter staining. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody. 

  • Immunofluorescence analysis of -20℃ acetone-fixed Jurkat cells labeling hnRNP U using ab180952 at a 1/100 dilution (green) with Dapi counter staining (blue). Goat anti rabbit IgG (Dylight 488), at a dilution of 1/200, was used as a secondary antibody.

  • Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling hnRNP U using ab180952 at a 1/100 dilution, or Rabbit monoclonal IgG as an isotype control.

References

This product has been referenced in:

See all 2 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab180952.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up