Anti-hnRNP U/p120 antibody (ab122906)
Key features and details
- Rabbit polyclonal to hnRNP U/p120
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
-
Product name
Anti-hnRNP U/p120 antibody
See all hnRNP U/p120 primary antibodies -
Description
Rabbit polyclonal to hnRNP U/p120 -
Host species
Rabbit -
Specificity
ab122906 can recognize exogenous Myc tagged hnRNP U/p120 (vector expressed hnRNP U/p120) and endogenous hnRNP U/p120 (native). -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 6
Preservative: 0.05% Sodium azide
Constituent: 99% Whole serum -
Concentration information loading...
-
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122906 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/3000. Predicted molecular weight: 91 kDa.
|
|
ICC/IF |
1/500 - 1/1000.
|
Notes |
---|
WB
1/1000 - 1/3000. Predicted molecular weight: 91 kDa. |
ICC/IF
1/500 - 1/1000. |
Target
-
Function
Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA. -
Sequence similarities
Contains 1 B30.2/SPRY domain.
Contains 1 SAP domain. -
Post-translational
modificationsExtensively phosphorylated.
Arg-733 and Arg-739 are dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Nucleus. Cytoplasm. Cell surface. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Also found associated with the cell surface. - Information by UniProt
-
Database links
- Entrez Gene: 3192 Human
- Entrez Gene: 51810 Mouse
- Omim: 602869 Human
- SwissProt: Q00839 Human
- SwissProt: Q8VEK3 Mouse
- Unigene: 106212 Human
- Unigene: 86589 Mouse
-
Alternative names
- Heterogeneous nuclear ribonucleoprotein U antibody
- hnRNP U antibody
- hnRNP U protein antibody
see all
Images
-
Lanes 1-2 : anti Myc antibody
Lanes 3-4 : Anti-hnRNP U/p120 antibody (ab122906) at 1/1000 dilution
Lanes 1 & 3 : lysates from SAOS2 cells transfected with pRc/CMV vector
Lanes 2 & 4 : lysates from SAOS2 cells transfected with pRc/CMV vector expressing Myc tagged hnRNP U
Predicted band size: 91 kDaMyc - hnRNP U/p120: Exogenous Myc tagged hnRNP U/p120
hnRNP U/p120: endogenous hnRNP U/p120. -
Immunofuorescent analysis of hnRNP U/p120 expression using ab122906 in Mouse P19 neurons (left) and in cells with in situ extracted nuclear matrix (right). Note that hnRNP U is concentrated in intranuclear speckles throughout the nucleoplasm.
Protocols
Datasheets and documents
-
Datasheet download
References (1)
ab122906 has been referenced in 1 publication.
- Taniura H & Yoshikawa K Necdin interacts with the ribonucleoprotein hnRNP U in the nuclear matrix. J Cell Biochem 84:545-55 (2002). PubMed: 11813259