Product nameAnti-hnRNP U/p120 antibody
See all hnRNP U/p120 primary antibodies
DescriptionRabbit polyclonal to hnRNP U/p120
Tested applicationsSuitable for: IHC-P, WBmore details
Unsuitable for: IP
Species reactivityReacts with: Mouse, Human
Synthetic peptide within Human hnRNP U/p120 aa 450-500. The exact sequence is proprietary.
Database link: Q00839
- IHC-P: Human breast carcinoma. Mouse squamous cell carcinoma. IP: HeLa whole cell lysate. WB: HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264141 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/2000 - 1/10000.|
FunctionComponent of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA.
Sequence similaritiesContains 1 B30.2/SPRY domain.
Contains 1 SAP domain.
Arg-733 and Arg-739 are dimethylated, probably to asymmetric dimethylarginine.
Cellular localizationNucleus. Cytoplasm. Cell surface. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Also found associated with the cell surface.
- Information by UniProt
- Heterogeneous nuclear ribonucleoprotein U antibody
- hnRNP U antibody
- hnRNP U protein antibody
All lanes : Anti-hnRNP U/p120 antibody (ab264141) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Lane 5 : NIH/3T3 whole cell lysate at 50 µg
Exposure time: 10 seconds
hnRNP U/p120 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264141 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264141 at 1 µg/ml.
Lane 1: ab264141 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 30 secs.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human breast carcinoma (left panel) and mouse squamous cell carcinoma (right panel) labeling hnRNP U/p120 with ab264141 at 1/200 dilution. Detection: DAB.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264141 has not yet been referenced specifically in any publications.