Product nameAnti-hnRNP U/p120 antibody [EPR12278(2)(B)]
See all hnRNP U/p120 primary antibodies
DescriptionRabbit monoclonal [EPR12278(2)(B)] to hnRNP U/p120
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human hnRNP U/p120 aa 50-150. The exact sequence is proprietary.
Database link: Q00839
- K562, Jurkat and 293 cell lines; Human clear cell carcinoma of kidney tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, 59% PBS
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab180952 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IHC-P||1/50 - 1/100.|
|WB||1/10000 - 1/50000. Predicted molecular weight: 91 kDa.|
FunctionComponent of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA.
Sequence similaritiesContains 1 B30.2/SPRY domain.
Contains 1 SAP domain.
Arg-733 and Arg-739 are dimethylated, probably to asymmetric dimethylarginine.
Cellular localizationNucleus. Cytoplasm. Cell surface. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Also found associated with the cell surface.
- Information by UniProt
- Heterogeneous nuclear ribonucleoprotein U antibody
- hnRNP U antibody
- hnRNP U protein antibody
All lanes : Anti-hnRNP U/p120 antibody [EPR12278(2)(B)] (ab180952) at 1/20000 dilution
Lane 1 : K562 cell lysate with NFDM/TBST
Lane 2 : Jurkat cell lysate with NFDM/TBST
Lane 3 : 293 cell lysate with NFDM/TBST
Lysates/proteins at 20 µg per lane.
Blocking peptides at 5 % per lane.
All lanes : Peroxidase conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 91 kDa
Immunohistochemical analysis of paraffin-embedded Human clear cell carcinoma of kidney labeling hnRNP U/p120 using ab180952 at a 1/100 dilution, with Hematoxylin counter staining. Prediluted (ready to use) HRP Polymer for Rabbit IgG was used as a secondary antibody.
Immunofluorescence analysis of -20℃ acetone-fixed Jurkat cells labeling hnRNP U/p120 using ab180952 at a 1/100 dilution (green) with Dapi counter staining (blue). Goat anti rabbit IgG (Dylight 488), at a dilution of 1/200, was used as a secondary antibody.
Flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling hnRNP U/p120 using ab180952 at a 1/100 dilution, or Rabbit monoclonal IgG as an isotype control.
This product has been referenced in:
- Meredith EK et al. An RNA matchmaker protein regulates the activity of the long noncoding RNA HOTAIR. RNA 22:995-1010 (2016). Read more (PubMed: 27146324) »
- Kishikawa T et al. Satellite RNAs promote pancreatic oncogenic processes via the dysfunction of YBX1. Nat Commun 7:13006 (2016). WB . Read more (PubMed: 27667193) »