Product nameAnti-hnRNP U/p120 antibody [EPR12279]
See all hnRNP U/p120 primary antibodies
DescriptionRabbit monoclonal [EPR12279] to hnRNP U/p120
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivityReacts with: Human
within Human hnRNP U/p120 aa 750 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q00839
- K562, Jurkat and 293T cell lysates; Human breast carcinoma and thyroid gland carcinoma tissues; HeLa cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab172608 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 91 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/250 - 1/500.|
FunctionComponent of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA.
Sequence similaritiesContains 1 B30.2/SPRY domain.
Contains 1 SAP domain.
Arg-733 and Arg-739 are dimethylated, probably to asymmetric dimethylarginine.
Cellular localizationNucleus. Cytoplasm. Cell surface. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Also found associated with the cell surface.
- Information by UniProt
- Heterogeneous nuclear ribonucleoprotein U antibody
- hnRNP U antibody
- hnRNP U protein antibody
All lanes : Anti-hnRNP U/p120 antibody [EPR12279] (ab172608) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 91 kDa
Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling hnRNP U/p120 with ab172608 at 1/50 dilution.
Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue labeling hnRNP U/p120 with ab172608 at 1/50 dilution.
Immunofluorescent staining of HeLa cells labeling hnRNP U/p120 with ab172608 at 1/250 dilution.
ab172608 has not yet been referenced specifically in any publications.