Overview

  • Product name
    Anti-hnRNP U/p120 antibody [EPR12279]
    See all hnRNP U/p120 primary antibodies
  • Description
    Rabbit monoclonal [EPR12279] to hnRNP U/p120
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    within Human hnRNP U/p120 aa 750 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q00839

  • Positive control
    • K562, Jurkat and 293T cell lysates; Human breast carcinoma and thyroid gland carcinoma tissues; HeLa cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12279
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172608 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 91 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Component of the CRD-mediated complex that promotes MYC mRNA stabilization. Binds to pre-mRNA. Has high affinity for scaffold-attached region (SAR) DNA. Binds to double- and single-stranded DNA and RNA.
    • Sequence similarities
      Contains 1 B30.2/SPRY domain.
      Contains 1 SAP domain.
    • Post-translational
      modifications
      Extensively phosphorylated.
      Arg-733 and Arg-739 are dimethylated, probably to asymmetric dimethylarginine.
    • Cellular localization
      Nucleus. Cytoplasm. Cell surface. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Component of ribonucleosomes. Also found associated with the cell surface.
    • Information by UniProt
    • Database links
    • Alternative names
      • Heterogeneous nuclear ribonucleoprotein U antibody
      • hnRNP U antibody
      • hnRNP U protein antibody
      • HNRNPU antibody
      • hnRNPU protein antibody
      • HNRPU antibody
      • HNRPU_HUMAN antibody
      • p120 antibody
      • p120 nuclear protein antibody
      • pp120 antibody
      • SAF A antibody
      • SAF-A antibody
      • SAFA antibody
      • Scaffold attachment factor A antibody
      • U21.1 antibody
      see all

    Images

    • All lanes : Anti-hnRNP U/p120 antibody [EPR12279] (ab172608) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 91 kDa

    • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling hnRNP U/p120 with ab172608 at 1/50 dilution.

    • Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue labeling hnRNP U/p120 with ab172608 at 1/50 dilution.

    • Immunofluorescent staining of HeLa cells labeling hnRNP U/p120 with ab172608 at 1/250 dilution.

    References

    ab172608 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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