Anti-HNRNPA0 antibody (ab157133)

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Overview

  • Product name

    Anti-HNRNPA0 antibody
    See all HNRNPA0 primary antibodies

  • Description

    Rabbit polyclonal to HNRNPA0

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan, Zebra finch, Xenopus tropicalis, Elephant

  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 150-200 of Human HNRNPA0 (NP_006796.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • 293T, HeLa, Jurkat, mouse TCMK 1 and mouse NIH 3T3 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab157133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 31 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • Western blot - Anti-HNRNPA0 antibody (ab157133)
    Western blot - Anti-HNRNPA0 antibody (ab157133)
    All lanes : Anti-HNRNPA0 antibody (ab157133) at 0.4 µg/ml

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TMCK 1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 31 kDa


    Exposure time: 10 seconds
  • Immunoprecipitation - Anti-HNRNPA0 antibody (ab157133)
    Immunoprecipitation - Anti-HNRNPA0 antibody (ab157133)
    Detection of HNRNPA0 by Western Blot of Immunprecipitate.
    ab157133 at 1µg/ml labeling HNRNPA0 in 293T whole cell lysate immunoprecipitated using ab157133 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab157133 has not yet been referenced specifically in any publications.

Publishing research using ab157133? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Western blot abreview for Anti-HNRNPA0 antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma cells)
Gel Running Conditions
Reduced Denaturing (4 - 12%)
Loading amount
50000 cells
Specification
Glioblastoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
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Submitted May 20 2019

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