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  1. Link

    hnrnpa0-antibody-ab157133.pdf

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Epigenetics and Nuclear Signaling DNA / RNA RNA Processing Splicing
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Anti-HNRNPA0 antibody (ab157133)

  • Datasheet
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  • Western blot - Anti-HNRNPA0 antibody (ab157133)
  • Immunoprecipitation - Anti-HNRNPA0 antibody (ab157133)

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Primary

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Secondary

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Protein

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  • Datasheet
  • References
  • Protocols

Overview

  • Product name

    Anti-HNRNPA0 antibody
    See all HNRNPA0 primary antibodies
  • Description

    Rabbit polyclonal to HNRNPA0
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan, Zebra finch, Xenopus tropicalis, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 150-200 of Human HNRNPA0 (NP_006796.1).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • 293T, HeLa, Jurkat, mouse TCMK 1 and mouse NIH 3T3 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab157133 was affinity purified using an epitope specific to HNRNPA0 immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Splicing
    • Epigenetics and Nuclear Signaling
    • Chromatin Binding Proteins
    • DNA / RNA binding

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
    • NIH 3T3 whole cell lysate (ab7179)
    • Jurkat whole cell lysate (ab7899)
    • 293T whole cell lysate (ab95494)
  • Recombinant Protein

    • Recombinant Human HNRNPA0 protein (ab132718)
  • Related Products

    • Recombinant Human HNRNPA0 protein (ab132718)

Applications

Our Abpromise guarantee covers the use of ab157133 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 31 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    This protein is a component of ribonucleosomes.
  • Sequence similarities

    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Arg-291 is dimethylated, probably to asymmetric dimethylarginine.
  • Cellular localization

    Nucleus. Component of ribonucleosomes.
  • Target information above from: UniProt accession Q13151 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 416302 Chicken
    • Entrez Gene: 745876 Chimpanzee
    • Entrez Gene: 540311 Cow
    • Entrez Gene: 481524 Dog
    • Entrez Gene: 10949 Human
    • Entrez Gene: 77134 Mouse
    • Entrez Gene: 498696 Rat
    • Omim: 609409 Human
    • SwissProt: Q13151 Human
    • SwissProt: Q9CX86 Mouse
    • Unigene: 645902 Human
    • Unigene: 96996 Human
    • Unigene: 490274 Mouse
    see all
  • Alternative names

    • Heterogeneous nuclear ribonucleoprotein A0 antibody
    • hnRNA binding protein antibody
    • hnRNP A0 antibody
    • HNRNPA0 antibody
    • HNRPA0 antibody
    • ROA0_HUMAN antibody
    see all

Images

  • Western blot - Anti-HNRNPA0 antibody (ab157133)
    Western blot - Anti-HNRNPA0 antibody (ab157133)
    All lanes : Anti-HNRNPA0 antibody (ab157133) at 0.4 µg/ml

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : TMCK 1 whole cell lysate
    Lane 5 : NIH 3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 31 kDa


    Exposure time: 10 seconds
  • Immunoprecipitation - Anti-HNRNPA0 antibody (ab157133)
    Immunoprecipitation - Anti-HNRNPA0 antibody (ab157133)
    Detection of HNRNPA0 by Western Blot of Immunprecipitate.
    ab157133 at 1µg/ml labeling HNRNPA0 in 293T whole cell lysate immunoprecipitated using ab157133 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Datasheets and documents

    • Datasheet
  • References

    ab157133 has not yet been referenced specifically in any publications.

    Publishing research using ab157133? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Western blot abreview for Anti-HNRNPA0 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Glioblastoma cells)
    Gel Running Conditions
    Reduced Denaturing (4 - 12%)
    Loading amount
    50000 cells
    Specification
    Glioblastoma cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1%
    Read More

    Abcam user community

    Verified customer

    Submitted May 20 2019

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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