- Datasheet
- Specific References
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Overview
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Product nameAnti-HNRNPA0 antibody
See all HNRNPA0 primary antibodies -
DescriptionRabbit polyclonal to HNRNPA0
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IPmore details
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Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan, Zebra finch, Xenopus tropicalis, Elephant
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 150-200 of Human HNRNPA0 (NP_006796.1).
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Positive control
- 293T, HeLa, Jurkat, mouse TCMK 1 and mouse NIH 3T3 whole cell lysates.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
Note: pH 7 to 8 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab157133 was affinity purified using an epitope specific to HNRNPA0 immobilized on solid support.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab157133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 31 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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FunctionThis protein is a component of ribonucleosomes.
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Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
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Post-translational
modificationsArg-291 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localizationNucleus. Component of ribonucleosomes.
- Information by UniProt
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Database links
- Entrez Gene: 416302 Chicken
- Entrez Gene: 745876 Chimpanzee
- Entrez Gene: 540311 Cow
- Entrez Gene: 481524 Dog
- Entrez Gene: 10949 Human
- Entrez Gene: 77134 Mouse
- Entrez Gene: 498696 Rat
- Omim: 609409 Human
see all -
Alternative names
- Heterogeneous nuclear ribonucleoprotein A0 antibody
- hnRNA binding protein antibody
- hnRNP A0 antibody
see all
Images
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Western blot - Anti-HNRNPA0 antibody (ab157133)All lanes : Anti-HNRNPA0 antibody (ab157133) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TMCK 1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 31 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-HNRNPA0 antibody (ab157133)Detection of HNRNPA0 by Western Blot of Immunprecipitate.
ab157133 at 1µg/ml labeling HNRNPA0 in 293T whole cell lysate immunoprecipitated using ab157133 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
References
ab157133 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"