Anti-HNRNPA0 antibody (ab157133)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-HNRNPA0 antibody
See all HNRNPA0 primary antibodies -
Description
Rabbit polyclonal to HNRNPA0 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan, Zebra finch, Xenopus tropicalis, Elephant -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 150-200 of Human HNRNPA0 (NP_006796.1).
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Positive control
- 293T, HeLa, Jurkat, mouse TCMK 1 and mouse NIH 3T3 whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab157133 was affinity purified using an epitope specific to HNRNPA0 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab157133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/5000. Predicted molecular weight: 31 kDa. | |
IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
This protein is a component of ribonucleosomes. -
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsArg-291 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Nucleus. Component of ribonucleosomes. - Information by UniProt
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Database links
- Entrez Gene: 416302 Chicken
- Entrez Gene: 745876 Chimpanzee
- Entrez Gene: 540311 Cow
- Entrez Gene: 481524 Dog
- Entrez Gene: 10949 Human
- Entrez Gene: 77134 Mouse
- Entrez Gene: 498696 Rat
- Omim: 609409 Human
see all -
Alternative names
- Heterogeneous nuclear ribonucleoprotein A0 antibody
- hnRNA binding protein antibody
- hnRNP A0 antibody
see all
Images
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All lanes : Anti-HNRNPA0 antibody (ab157133) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TMCK 1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 31 kDa
Exposure time: 10 seconds -
Detection of HNRNPA0 by Western Blot of Immunprecipitate.
ab157133 at 1µg/ml labeling HNRNPA0 in 293T whole cell lysate immunoprecipitated using ab157133 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
Datasheets and documents
References
ab157133 has not yet been referenced specifically in any publications.