Key features and details
- Rabbit polyclonal to HNRNPA0
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-HNRNPA0 antibody
See all HNRNPA0 primary antibodies
DescriptionRabbit polyclonal to HNRNPA0
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan, Zebra finch, Xenopus tropicalis, Elephant
- 293T, HeLa, Jurkat, mouse TCMK 1 and mouse NIH 3T3 whole cell lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab157133 was affinity purified using an epitope specific to HNRNPA0 immobilized on solid support.
Our Abpromise guarantee covers the use of ab157133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 31 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionThis protein is a component of ribonucleosomes.
Sequence similaritiesContains 2 RRM (RNA recognition motif) domains.
modificationsArg-291 is dimethylated, probably to asymmetric dimethylarginine.
Cellular localizationNucleus. Component of ribonucleosomes.
- Information by UniProt
- Heterogeneous nuclear ribonucleoprotein A0 antibody
- hnRNA binding protein antibody
- hnRNP A0 antibody
All lanes : Anti-HNRNPA0 antibody (ab157133) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TMCK 1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 31 kDa
Exposure time: 10 seconds
Detection of HNRNPA0 by Western Blot of Immunprecipitate.
ab157133 at 1µg/ml labeling HNRNPA0 in 293T whole cell lysate immunoprecipitated using ab157133 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
ab157133 has not yet been referenced specifically in any publications.