Anti-HNRNPA0 antibody (ab157133)
Key features and details
- Rabbit polyclonal to HNRNPA0
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HNRNPA0 antibody
See all HNRNPA0 primary antibodies -
Description
Rabbit polyclonal to HNRNPA0 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset, Orangutan, Zebra finch, Xenopus tropicalis, Elephant
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Immunogen
Synthetic peptide, corresponding to a region within amino acids 150-200 of Human HNRNPA0 (NP_006796.1).
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Positive control
- 293T, HeLa, Jurkat, mouse TCMK 1 and mouse NIH 3T3 whole cell lysates.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab157133 was affinity purified using an epitope specific to HNRNPA0 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab157133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 31 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
This protein is a component of ribonucleosomes. -
Sequence similarities
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsArg-291 is dimethylated, probably to asymmetric dimethylarginine. -
Cellular localization
Nucleus. Component of ribonucleosomes. - Information by UniProt
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Database links
- Entrez Gene: 416302 Chicken
- Entrez Gene: 745876 Chimpanzee
- Entrez Gene: 540311 Cow
- Entrez Gene: 481524 Dog
- Entrez Gene: 10949 Human
- Entrez Gene: 77134 Mouse
- Entrez Gene: 498696 Rat
- Omim: 609409 Human
see all -
Alternative names
- Heterogeneous nuclear ribonucleoprotein A0 antibody
- hnRNA binding protein antibody
- hnRNP A0 antibody
see all
Images
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Western blot - Anti-HNRNPA0 antibody (ab157133)All lanes : Anti-HNRNPA0 antibody (ab157133) at 0.4 µg/ml
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TMCK 1 whole cell lysate
Lane 5 : NIH 3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 31 kDa
Exposure time: 10 seconds -
Immunoprecipitation - Anti-HNRNPA0 antibody (ab157133)Detection of HNRNPA0 by Western Blot of Immunprecipitate.
ab157133 at 1µg/ml labeling HNRNPA0 in 293T whole cell lysate immunoprecipitated using ab157133 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
Protocols
Datasheets and documents
References (0)
ab157133 has not yet been referenced specifically in any publications.
