Overview

  • Product name

    Anti-HNRPAB antibody [EPR16944]
    See all HNRPAB primary antibodies
  • Description

    Rabbit monoclonal [EPR16944] to HNRPAB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human HNRPAB aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q99729

  • Positive control

    • WB: K562, Jurkat, HeLa, C6, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Human fetal brain lysate; Mouse brain and rat brain lysates. IHC-P: Human cervix carcinoma, mouse liver and rat cardiac muscle tissues. ICC/IF: Jurkat cells. IP: K562 whole cell lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab199724 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 36 kDa).
ICC/IF 1/500.
Flow Cyt Use a concentration of 1 µg/ml.

Target

  • Function

    Binds single-stranded RNA. Has a high affinity for G-rich and U-rich regions of hnRNA. Also binds to APOB mRNA transcripts around the RNA editing site.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications

    Dimethylation at Arg-322 is probably asymmetric.
  • Cellular localization

    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links

  • Alternative names

    • ABBP 1 antibody
    • ABBP-1 antibody
    • ABBP1 antibody
    • Apobec 1 binding protein 1 antibody
    • APOBEC1 binding protein 1 antibody
    • APOBEC1-binding protein 1 antibody
    • Apolipoprotein B mRNA editing enzyme catalytic polypeptide 1- protein 1 antibody
    • FLJ40338 antibody
    • Heterogeneous nuclear ribonucleoprotein A/B antibody
    • hnRNP A/B antibody
    • hnRNP type A/B protein antibody
    • HNRNPAB antibody
    • HNRPAB antibody
    • ROAA_HUMAN antibody
    see all

Images

  • All lanes : Anti-HNRPAB antibody [EPR16944] (ab199724) at 1/5000 dilution

    Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    The expression profile observed is consistent with what has been described in the literature PMID: 22332140.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • ab199724 staining HNRPAB in Jurkat (human acute T cell leukemia) cells by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/2700. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Anti-HNRPAB antibody [EPR16944] (ab199724) at 1/5000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    The expression profile observed is consistent with what has been described in the literature PMID: 22332140.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-HNRPAB antibody [EPR16944] (ab199724) at 1/1000 dilution

    Lane 1 : Mouse brain lysates
    Lane 2 : Rat brain lysates
    Lane 3 : C6 (Rat glial tumor cells) whole cell lysate
    Lane 4 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 5 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 6 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 36 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    The expression profile observed is consistent with what has been described in the literature PMID: 22332140.

    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling HNRPAB with ab199724 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on Human cervix carcinoma tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

  • Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling HNRPAB with ab199724 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on mouse liver tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

  • Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling HNRPAB with ab199724 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Nuclear staining on rat cardiac muscle tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051).

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling HNRPAB with ab199724 at 1/500 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Nuclear staining on Jurkat cell line is observed. The nuclear counterstain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
    The negative controls are as follows:-
    -ve control 1: ab199724 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
    -ve control 2: ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/500 dilution.

  • HNRPAB was immunoprecipitated from 1mg of K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate with ab199724 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199724 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: K562 whole cell lysate 10 µg (Input). Lane 2: ab199724 IP in K562 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199724 in K562 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

ab199724 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Xenopus laevis Cell lysate - other (oocytes)
Gel Running Conditions
Reduced Denaturing (8% TrisGlycine Gel)
Loading amount
0.1 µg
Specification
oocytes
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C

Ms. Samantha Jeschonek

Verified customer

Submitted Jan 09 2018

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