Application
Western blot
Sample
Xenopus laevis Cell lysate - other (oocytes)
Gel Running Conditions
Reduced Denaturing (8% TrisGlycine Gel)
Loading amount
0.1 µg
Specification
oocytes
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Blocking Agent
Dilution
1/1000
Detection
Detection method
SuperSignal West Pico PLUS Cehmiluminesc
Exposure
40 second(s)
Bands
Specific: 40 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Samantha Jeschonek
Verified customer
Submitted Jan 09 2018