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Synthetic peptide within Human HNRPAB aa 8-38 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: Q99729
Our Abpromise guarantee covers the use of ab174971 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/10 - 1/50.|
|WB||1/100 - 1/500. Predicted molecular weight: 36 kDa.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Immunohistochemical analysis of formalin fixed, paraffin embedded Human cervix carcinoma labeling HNRPAB with ab174971 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Immunofluorescence confocal analysis of MCF7 cells labeling HNRPAB with ab174971 at 1/25 dilution. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab174971 (1/25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1/400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HNRPAB immunoreactivity is localized to Nucleus significantly.
ab174971 has not yet been referenced specifically in any publications.
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