Overview

  • Product name
    Anti-HNRPAB antibody - N-terminal
    See all HNRPAB primary antibodies
  • Description
    Rabbit polyclonal to HNRPAB - N-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HNRPAB aa 8-38 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q99729

  • Positive control
    • HepG2 cell line lysate; Human cervix carcinoma tissue; MCF7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab174971 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/10 - 1/50.
WB 1/100 - 1/500. Predicted molecular weight: 36 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function
    Binds single-stranded RNA. Has a high affinity for G-rich and U-rich regions of hnRNA. Also binds to APOB mRNA transcripts around the RNA editing site.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Dimethylation at Arg-322 is probably asymmetric.
  • Cellular localization
    Nucleus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
  • Information by UniProt
  • Database links
  • Alternative names
    • ABBP 1 antibody
    • ABBP-1 antibody
    • ABBP1 antibody
    • Apobec 1 binding protein 1 antibody
    • APOBEC1 binding protein 1 antibody
    • APOBEC1-binding protein 1 antibody
    • Apolipoprotein B mRNA editing enzyme catalytic polypeptide 1- protein 1 antibody
    • FLJ40338 antibody
    • Heterogeneous nuclear ribonucleoprotein A/B antibody
    • hnRNP A/B antibody
    • hnRNP type A/B protein antibody
    • HNRNPAB antibody
    • HNRPAB antibody
    • ROAA_HUMAN antibody
    see all

Images

  • Anti-HNRPAB antibody - N-terminal (ab174971) at 1/100 dilution + HepG2 cell line lysate at 35 µg

    Secondary
    HRP anti-rabbit at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 36 kDa

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human cervix carcinoma labeling HNRPAB with ab174971 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Immunofluorescence confocal analysis of MCF7 cells labeling HNRPAB with ab174971 at 1/25 dilution. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab174971  (1/25, 1 h at 37°C). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1/400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). HNRPAB immunoreactivity is localized to Nucleus significantly.

References

ab174971 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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