Overview

  • Product name
  • Description
    Rabbit polyclonal to HNRPDL
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Xenopus tropicalis
  • Immunogen

    Synthetic peptide within Human HNRPDL aa 214-244 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary. NP_112740.1.
    Database link: O14979

  • Positive control
    • K562, HepG2 and Jurkat cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab175067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 46 kDa.

Target

  • Function
    Acts as a transcriptional regulator. Promotes transcription repression. Promotes transcription activation in differentiated myotubes (By similarity). Binds to double- and single-stranded DNA sequences. Binds to the transcription suppressor CATR sequence of the COX5B promoter (By similarity). Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Binds both to nuclear and cytoplasmic poly(A) mRNAs. Binds to poly(G) and poly(A), but not to poly(U) or poly(C) RNA homopolymers. Binds to the 5'-ACUAGC-3' RNA consensus sequence.
  • Tissue specificity
    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Expressed in myeloid leukemia, gastric adenocarcinoma, cervical carcinoma, hepatoma, fibrosarcoma, colon adenocarcinoma, epidermoid carcinoma, osteosarcoma and urinary bladder carcinoma cells.
  • Sequence similarities
    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications
    Dimethylation of Arg-408 is probably of the asymmetric type.
  • Cellular localization
    Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm in a TNPO1-dependent manner.
  • Information by UniProt
  • Database links
  • Alternative names
    • A+U-rich element RNA binding factor antibody
    • AA407431 antibody
    • AA959857 antibody
    • AU rich element RNA binding factor antibody
    • AU-rich element RNA-binding factor antibody
    • D5Ertd650e antibody
    • D5Wsu145e antibody
    • Heterogeneous nuclear ribonucleoprotein D like antibody
    • Heterogeneous nuclear ribonucleoprotein D like protein antibody
    • Heterogeneous nuclear ribonucleoprotein D-like antibody
    • hnHNRP DL antibody
    • HNRDL_HUMAN antibody
    • HNRNP antibody
    • hnRNP D-like antibody
    • hnRNP DL antibody
    • HNRNPDL antibody
    • hnRPD like protein antibody
    • JKT41 binding protein antibody
    • JKT41-binding protein antibody
    • JKTBP antibody
    • JKTBP2 antibody
    • laAUF1 antibody
    • MGC125262 antibody
    • Protein laAUF1 antibody
    see all

Images

  • All lanes : Anti-HNRPDL antibody (ab175067) at 1/100 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Jurkat cell lysate

    Lysates/proteins at 35 µg per lane.

    Predicted band size: 46 kDa

References

ab175067 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (fibroblast)
Permeabilization
Yes - 0.3% Triton X-100 in blocking buffer
Specification
fibroblast
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 01 2018

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Lymphocytes)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
30 µg
Specification
Lymphocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 25 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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