Immunocytochemistry/ Immunofluorescence abreview for Anti-HNRPDL antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (fibroblast)
Permeabilization
Yes - 0.3% Triton X-100 in blocking buffer
Specification
fibroblast
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA/ 0.3% Triton X-100 in PBS
Dilution
1/50

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-rabbit IgG (H+L), F(ab')2 Fragment (Alexa Flu
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 555

Additional data

Abcam response
Thank you for submitting this AbReview and your valuable feedback. We are pleased that this antibody worked well for you in ICC, an applicationthat has not been tested before. We welcome any further feedback and AbReviews from other researchers who have used this antibody in ICC which will help us assess how well the antibody works in this application.

Abcam user community

Verified customer

Submitted Feb 01 2018

Sign up