Recombinant Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (ab179439)
![Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (ab179439)](https://www.abcam.com/ps/products/179/ab179439/Images/ab179439-207519-anti-hnrph2-antibody-epr12170b-western-blot.jpg "Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (ab179439)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12170(B)] to HNRPH2/HNRNPH2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)]
See all HNRPH2/HNRNPH2 primary antibodies -
Description
Rabbit monoclonal [EPR12170(B)] to HNRPH2/HNRNPH2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human HNRPH2/HNRNPH2 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P55795 -
Positive control
- 293T and MCF-7 cell lysates; MCF-7 cells.
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General notes
This product was previously labelled as HNRPH2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR12170(B) -
Isotype
IgG
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab179439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Predicted molecular weight: 49 kDa. | |
| ICC/IF | 1/100 - 1/250. |
Target
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Function
This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Binds poly(RG). -
Tissue specificity
Expressed ubiquitously. -
Sequence similarities
Contains 3 RRM (RNA recognition motif) domains. -
Cellular localization
Nucleus > nucleoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3188 Human
- Entrez Gene: 56258 Mouse
- Entrez Gene: 308650 Rat
- Omim: 300610 Human
- SwissProt: P55795 Human
- SwissProt: P70333 Mouse
- SwissProt: Q6AY09 Rat
- Unigene: 432485 Human
see all -
Alternative names
- FTP 3 antibody
- FTP-3 antibody
- FTP3 antibody
see all
Images
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Western blot - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (ab179439)All lanes : Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (ab179439) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : MCF-7 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 49 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (ab179439)](https://www.abcam.com/ps/products/179/ab179439/Images/ab179439-207518-anti-hnrph2-antibody-epr12170b-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (ab179439)Immunofluorescent analysis of MCF-7 cells labeling HNRPH2/HNRNPH2 with ab179439 at 1/100 dilution.
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![Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (ab179439)](https://www.abcam.com/ps/products/179/ab179439/Images/ab179439-3-benefits-of-recombinant-antibodies.png)
Anti-HNRPH2/HNRNPH2 antibody [EPR12170(B)] (ab179439)
Protocols
Datasheets and documents
References (1)
ab179439 has been referenced in 1 publication.
- Xu C et al. HnRNP F/H associate with hTERC and telomerase holoenzyme to modulate telomerase function and promote cell proliferation. Cell Death Differ 27:1998-2013 (2020). PubMed: 31863069
