Product nameAnti-HNRPH2/HNRNPH2 antibody [EPR12171]
See all HNRPH2/HNRNPH2 primary antibodies
DescriptionRabbit monoclonal [EPR12171] to HNRPH2/HNRNPH2
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
within Human HNRPH2/HNRNPH2 aa 350 to the C-terminus. The exact sequence is proprietary.
Database link: P55795
- 293, Jurkat and K562. Human Endometrium tissue. 293 cells.
This product was previously labelled as HNRPH2
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181171 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).|
|ICC/IF||1/50 - 1/100.|
FunctionThis protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Binds poly(RG).
Tissue specificityExpressed ubiquitously.
Sequence similaritiesContains 3 RRM (RNA recognition motif) domains.
Cellular localizationNucleus > nucleoplasm.
- Information by UniProt
- FTP 3 antibody
- FTP-3 antibody
- FTP3 antibody
All lanes : Anti-HNRPH2/HNRNPH2 antibody [EPR12171] (ab181171) at 1/10000 dilution
Lane 1 : 293 Cell lysate
Lane 2 : Jurkat Cell lysate
Lane 3 : K562 Cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 49 kDa
Immunofluorescent analysis of 4% paraformaldehyde fixed 293 cells labeling HNRPH2/HNRNPH2 with ab181171 at a 1/100 dilution (green). Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) at a 1/200 dilution. Right panel conterstained with Dapi blue.
Immunohistochemical analysis of paraffin-embedded Human Endometrium tissue labeling HNRPH2/HNRNPH2 with ab181171at 1/250 dilution. Secondary antibody:anti rabbit IgG (HRP) prediluted. Counter stain Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ab181171 has not yet been referenced specifically in any publications.