Product nameAnti-HNRPH3 antibody
DescriptionRabbit polyclonal to HNRPH3
Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
- Raji cell lysate. Human kidney tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab66663 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
RelevanceHNRPH3 belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are RNA binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport.
- 2H9 antibody
- FLJ34092 antibody
- Heterogeneous nuclear ribonucleoprotein H3 antibody
All lanes : Anti-HNRPH3 antibody (ab66663) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Raji cell lysate at 10 µg
All lanes : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 37 kDa
Observed band size: 37 kDa
Additional bands at: 17 kDa, 35 kDa (possible isoform). We are unsure as to the identity of these extra bands.
Gel concentration: 12%
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of human kidney tissue labelling HNRPH3 with ab66663 at 4-8µg/ml. Arrows indicate positively labelled epithelial cells of the renal tubule. Magnifciation: 400X.
ab66663 has not yet been referenced specifically in any publications.