Key features and details
- Rabbit polyclonal to HO-2
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HO-2 antibody
See all HO-2 primary antibodies
DescriptionRabbit polyclonal to HO-2
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human HO-2 aa 1-50. The exact sequence is proprietary.
Database link: P30519
- IP: HO-2 IP in HEK-293T whole cell lysate.
ab245605 has not performed satisfactorily when used for WB of HO-2 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of HO-2.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245605 was affinity purified using an epitope specific to HO-2 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245605 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionHeme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.
Sequence similaritiesBelongs to the heme oxygenase family.
Contains 2 HRM (heme regulatory motif) repeats.
Cellular localizationMicrosome. Endoplasmic reticulum.
- Information by UniProt
- Heme oxygenase (decycling) 2 antibody
- Heme oxygenase (decyclizing) 2 antibody
- Heme oxygenase 2 antibody
HO-2 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) using ab245605 at 6 μg/reaction. Western blot was performed on the immunoprecipitate using ab245605 at 1 μg/ml.
Lane 1: ab245605 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG in HEK-293T whole cell lysate.
Detection: Chemiluminescence with an exposure time of 10 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245605 has not yet been referenced specifically in any publications.