Anti-HO-2 antibody (ab90515)
Key features and details
- Rabbit polyclonal to HO-2
- Suitable for: WB, ICC/IF, IHC-P, IP, ELISA
- Reacts with: Mouse, Rat, Hamster, Dog, Human, Pig, Monkey
- Isotype: IgG
Overview
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Product name
Anti-HO-2 antibody
See all HO-2 primary antibodies -
Description
Rabbit polyclonal to HO-2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-P, IP, ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat, Hamster, Dog, Human, Pig, Monkey -
Immunogen
Synthetic peptide corresponding to Human HO-2 (N terminal).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab90515 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
1/1000. Predicted molecular weight: 36 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000. Predicted molecular weight: 36 kDa. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
Target
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Function
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Heme oxygenase 2 could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter. -
Sequence similarities
Belongs to the heme oxygenase family.
Contains 2 HRM (heme regulatory motif) repeats. -
Cellular localization
Microsome. Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 479864 Dog
- Entrez Gene: 3163 Human
- Entrez Gene: 15369 Mouse
- Entrez Gene: 79239 Rat
- Omim: 141251 Human
- SwissProt: P30519 Human
- SwissProt: O70252 Mouse
- SwissProt: P23711 Rat
see all -
Alternative names
- Heme oxygenase (decycling) 2 antibody
- Heme oxygenase (decyclizing) 2 antibody
- Heme oxygenase 2 antibody
see all
Images
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ab90515 staining HO-2 in MCF7 cells by Immunofluorescence (green). Nuclei are stained in blue pseudocolor using DRAQ5.
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All lanes : Anti-HO-2 antibody (ab90515) at 1/1000 dilution
Lane 1 : HO-2 Recombinant Human Protein
Lane 2 : Human Liver Microsome Extract
Lane 3 : Mouse Liver Microsome Extract
Lane 4 : Rat Liver Microsome Extract
Lane 5 : Dog Liver Microsome Extract
Predicted band size: 36 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab90515 has been referenced in 5 publications.
- Hanna DA et al. Heme oxygenase-2 (HO-2) binds and buffers labile ferric heme in human embryonic kidney cells. J Biol Chem 298:101549 (2022). PubMed: 34973332
- Patra U et al. Progressive Rotavirus Infection Downregulates Redox-Sensitive Transcription Factor Nrf2 and Nrf2-Driven Transcription Units. Oxid Med Cell Longev 2020:7289120 (2020). PubMed: 32322337
- Liu L et al. Heme oxygenase-2 is post-translationally regulated by heme occupancy in the catalytic site. J Biol Chem 295:17227-17240 (2020). PubMed: 33051205
- Yang F et al. Suppression of TRPV4 channels ameliorates anti-dipsogenic effects under hypoxia in the subfornical organ of rats. Sci Rep 6:30168 (2016). WB . PubMed: 27436489
- Lee S et al. Chronic Stress Decreases Cerebrovascular Responses During Rat Hindlimb Electrical Stimulation. Front Neurosci 9:462 (2015). WB ; Rat . PubMed: 26778944