Overview

  • Product name
    Hoechst 33258 Staining Dye Solution
  • Sample type
    Adherent cells, Suspension cells
  • Product overview

    Hoechst 33258 Staining Dye Solution (ab228550) is a fluorescent stain for labeling DNA in fluorescence microscopy.


    This product may be used in fluorescence microscopy, microplate, cuvette and flow cytometry applications. It can also be used to detect the contents of a sample DNA by plotting a standard emission-to-content curve.


    Molecular Weight: 533.88 


    Spectral Properties: Excitation = 352 nm; Fluorescence = 461 nm

  • Notes

    The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Two of these closely related bis-benzimides are commonly used: Hoechst 33258 and Hoechst 33342. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescence light around an emission maximum at 461 nm. The Hoechst stains may be used on live or fixed cells and are often used as a substitute for another nucleic acid stain (DAPI). The key difference between them is that the additional ethyl group of Hoechst 33342 renders it more lipophilic, and thus more able to cross intact cell membranes. In some applications, Hoechst 33258 is significantly less permeant.

  • Tested applications
    Suitable for: FM, Flow Cytmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 5 ml
    Hoechst 33258 (20 mM solution in water) 1 x 5ml

Applications

Our Abpromise guarantee covers the use of ab228550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Images

Protocols

References

ab228550 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228550.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up