Product nameHoechst 33258 Staining Dye Solution
Sample typeAdherent cells, Suspension cells
Hoechst 33258 Staining Dye Solution (ab228550) is a fluorescent stain for labeling DNA in fluorescence microscopy.
This product may be used in fluorescence microscopy, microplate, cuvette and flow cytometry applications. It can also be used to detect the contents of a sample DNA by plotting a standard emission-to-content curve.
Molecular Weight: 533.88
Spectral Properties: Excitation = 352 nm; Fluorescence = 461 nm
The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Two of these closely related bis-benzimides are commonly used: Hoechst 33258 and Hoechst 33342. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescence light around an emission maximum at 461 nm. The Hoechst stains may be used on live or fixed cells and are often used as a substitute for another nucleic acid stain (DAPI). The key difference between them is that the additional ethyl group of Hoechst 33342 renders it more lipophilic, and thus more able to cross intact cell membranes. In some applications, Hoechst 33258 is significantly less permeant.
Tested applicationsSuitable for: FM, Flow Cytmore details
Storage instructionsStore at -20°C. Please refer to protocols.
Components 5 ml Hoechst 33258 (20 mM solution in water) 1 x 5ml
Our Abpromise guarantee covers the use of ab228550 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FM||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
ab228550 has been referenced in 1 publication.
- Vasavda C et al. Bilirubin Links Heme Metabolism to Neuroprotection by Scavenging Superoxide. Cell Chem Biol 26:1450-1460.e7 (2019). PubMed: 31353321