Overview

  • Product name
    Hoechst 33342 Staining Dye Solution
  • Sample type
    Adherent cells, Suspension cells
  • Product overview

    Hoechst 33342 Staining Dye Solution (ab228551) is a fluorescent stain for labeling DNA in fluorescence microscopy. This product may be used in fluorescence microscopy, microplate, cuvette, and flow cytometry applications. It can also be used to detect the contents of a sample DNA by plotting a standard emission-tocontent curve.


    Molecular Weight: 561.93


    Spectral Properties: Excitation = 350 nm; Fluorescence = 461 nm.

  • Notes

    The Hoechst stains are a family of fluorescent stains for labeling DNA in fluorescence microscopy. Because these fluorescent stains label DNA, they are also commonly used to visualize nuclei and mitochondria. Two of these closely related bis-benzimides are commonly used: Hoechst 33258 and Hoechst 33342. Both dyes are excited by ultraviolet light at around 350 nm, and both emit blue/cyan fluorescence light with an emission maximum at 461 nm. The Hoechst stains may be used on live or fixed cells, and are often used as a substitute for another nucleic acid stain, DAPI. The key difference between them is that the additional ethyl group of Hoechst 33342 makes it more lipophilic, and thus more readily to cross intact cell membranes. In some applications, Hoechst 33258 is significantly less permeant than Hoechst 33342.

  • Tested applications
    Suitable for: FM, Flow Cytmore details

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 5 ml
    Hoechst 33342 (20 mM Solution in Water) 1 x 5ml

Applications

Our Abpromise guarantee covers the use of ab228551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Images

Protocols

References

This product has been referenced in:
  • Liu W  et al. CX3CL1 promotes lung cancer cell migration and invasion via the Src/focal adhesion kinase signaling pathway. Oncol Rep 41:1911-1917 (2019). Read more (PubMed: 30628679) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab228551.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up