Overview

  • Product name

    Anti-Homeobox protein cut-like 1 antibody
    See all Homeobox protein cut-like 1 primary antibodies
  • Description

    Rabbit polyclonal to Homeobox protein cut-like 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse Homeobox protein cut-like 1 aa 750-850 conjugated to keyhole limpet haemocyanin.
    Database link: P53564

  • Positive control

    • IHC-P: Mouse kidney and mouse brain FFPE tissue sections

Properties

Applications

Our Abpromise guarantee covers the use of ab140042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    Function: Probably has a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator (By similarity). Similarity: Belongs to the CUT homeobox family.
  • Cellular localization

    Nucleus
  • Database links

  • Alternative names

    • CCAAT displacement protein antibody
    • CDP antibody
    • CDP/Cut antibody
    • CDP1 antibody
    • Clox antibody
    • COY1 antibody
    • Cut homeobox antibody
    • Cut homolog antibody
    • Cut like 1 antibody
    • Cut like homeobox 1 antibody
    • CUTL1 antibody
    • CUX antibody
    • Cux/CDP antibody
    • CUX1 antibody
    • Golgi integral membrane protein 6 antibody
    • GOLIM6 antibody
    • Homeobox protein cux 1 antibody
    • Nbla10317 antibody
    • p100 antibody
    • p110 antibody
    • p200 antibody
    • p75 antibody
    • Putative protein product of Nbla10317 antibody
    see all

Images

  • IHC image of ab140042 staining in mouse brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab140042, 1µg/ml, for 15 mins at room temperature. A goat anti-rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Lutz D  et al. Proteolytic cleavage of transmembrane cell adhesion molecule L1 by extracellular matrix molecule Reelin is important for mouse brain development. Sci Rep 7:15268 (2017). Read more (PubMed: 29127326) »
See 1 Publication for this product

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