Anti-Homeobox protein cut-like 1 antibody (ab73885)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Homeobox protein cut-like 1 antibody
See all Homeobox protein cut-like 1 primary antibodies -
Description
Rabbit polyclonal to Homeobox protein cut-like 1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide corresponding to Human Homeobox protein cut-like 1 (N terminal).
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Positive control
- WB: Extracts from K562 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab73885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | 1/500 - 1/1000. Predicted molecular weight: 164 kDa. | |
ELISA | 1/40000. |
Target
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Relevance
Function: Probably has a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator (By similarity). Similarity: Belongs to the CUT homeobox family. -
Cellular localization
Nucleus -
Database links
- Entrez Gene: 1523 Human
- Omim: 116896 Human
- SwissProt: P39880 Human
- SwissProt: P70403 Mouse
- Unigene: 191482 Human
- Unigene: 320317 Mouse
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Alternative names
- CCAAT displacement protein antibody
- CDP antibody
- CDP/Cut antibody
see all
Images
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All lanes : Anti-Homeobox protein cut-like 1 antibody (ab73885) at 1/500 dilution
Lane 1 : extracts from K562 cells
Lane 2 : extracts from K562 cells with immunizing peptide at 10 µg
Lysates/proteins at 20 µg per lane.
Predicted band size: 164 kDa
Observed band size: 164 kDa
Additional bands at: 75 kDa, 80 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Wong CC et al. Inactivating CUX1 mutations promote tumorigenesis. Nat Genet 46:33-8 (2014). WB . Read more (PubMed: 24316979) »