Overview

  • Product name

    Anti-Homeobox protein cut-like 1 antibody
    See all Homeobox protein cut-like 1 primary antibodies
  • Description

    Rabbit polyclonal to Homeobox protein cut-like 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human Homeobox protein cut-like 1 (N terminal).

  • Positive control

    • WB: Extracts from K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab73885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 164 kDa.
ELISA 1/40000.

Target

  • Relevance

    Function: Probably has a broad role in mammalian development as a repressor of developmentally regulated gene expression. May act by preventing binding of positively-activing CCAAT factors to promoters. Component of nf-munr repressor; binds to the matrix attachment regions (MARs) (5' and 3') of the immunoglobulin heavy chain enhancer. Represses T-cell receptor (TCR) beta enhancer function by binding to MARbeta, an ATC-rich DNA sequence located upstream of the TCR beta enhancer. Binds to the TH enhancer; may require the basic helix-loop-helix protein TCF4 as a coactivator (By similarity). Similarity: Belongs to the CUT homeobox family.
  • Cellular localization

    Nucleus
  • Database links

  • Alternative names

    • CCAAT displacement protein antibody
    • CDP antibody
    • CDP/Cut antibody
    • CDP1 antibody
    • Clox antibody
    • COY1 antibody
    • Cut homeobox antibody
    • Cut homolog antibody
    • Cut like 1 antibody
    • Cut like homeobox 1 antibody
    • CUTL1 antibody
    • CUX antibody
    • Cux/CDP antibody
    • CUX1 antibody
    • Golgi integral membrane protein 6 antibody
    • GOLIM6 antibody
    • Homeobox protein cux 1 antibody
    • Nbla10317 antibody
    • p100 antibody
    • p110 antibody
    • p200 antibody
    • p75 antibody
    • Putative protein product of Nbla10317 antibody
    see all

Images

  • All lanes : Anti-Homeobox protein cut-like 1 antibody (ab73885) at 1/500 dilution

    Lane 1 : extracts from K562 cells
    Lane 2 : extracts from K562 cells with immunizing peptide at 10 µg

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 164 kDa
    Observed band size: 164 kDa
    Additional bands at: 75 kDa, 80 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Question
Answer

DISCOUNT CODE: ABTRIAL-00000
Ablaufdatum: 18. Februar 2013
Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab73885 in IP zu benutzen und dann eine Abbildung einzusenden, annehmen möchten. Wie am Telefon besprochen, können Sie den Gutscheincode zum Kauf weitere Produkte einsetzen, sobald Sie uns ein Abreview mit Abbildung für diesen Antikörper eingereicht haben.
Der Discountcode hat die Nummer ABTRIAL-00000 , deckt den Wert von ab73885 ab und kann zum Kauf weiterer Produkte eingesetzt werden.
Der Code wird gültig, sobald Sie uns ein Abreview mit Abbildung für diesen Antikörper über den Test in IP eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unseren Kundendienst mit dem Code und der ursprünglichen Bestellnummer.
Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview mit Abbildung und wünschen Ihnen viel Glück für Ihre Forschung.
Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

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Answer

Thank you for contacting Abcam.
You have to use the discount code by the expiration date that was sent with the discount codes. If you have any trouble using the code while trying to make a purchase, please contact our customer service department on 888-772-2226.
Also we only give out testing discount codes for antibodies that you actually purchase, we cannot give a testing code out for an antibody that you are getting using a testing discount code.
Since you have 3 discount codes but 5 antibodies you would like to get, I can give you a testing discount code for 2 of them.
Please let me know which two you would like the discount codes for and I will see if they are eligible.
I look forward to your response.

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Application
Immunohistochemistry (Frozen sections)
Sample
Zebra finch Tissue sections (embryonal forebrain)
Specification
embryonal forebrain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% TX100 in PBS
Blocking step
Roti-Immunoblock as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C

Dr. Robert Storm

Verified customer

Submitted Feb 06 2012

Answer

Thank you for contacting Abcam. I can issue you 3 testing discount codes to test the antibodies in finch tissue. We like to issue codes before the antibody has been purchased, but I can make an exception in this case. Below are the discount codes and instructions, when using the discount code, please be sure to use the code that goes with the particular antibody. If there is anything else I can help with, please let me know and I hope the experiments go well. Testing Discount Codes: DISCOUNT CODE: ab82901 - ****** ab51502 - ******ab61488 - ****** Expiration date: ******, this is for all of the codes I am very pleased to hear you would like to accept our offer and test ab82901/51502/61488 in finch tissue. This code will give you 1 free primary antibody for each code before the expiration date. To redeem this offer, please submit an Abreview for each antibody on finch tissue and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting us and sorry for the delay in getting back to you. We have a number of collaborators which produce antibodies for us as well as in house production of antibodies. The 5 antibodies that we have against CUTL1 have all been sourced through collaborators. All of the antibodies have been characterised through Western blotting and ab54583 and ab11584 has also been used in IHC. The diffferences observed in the western blots may be due to the antibodies being produced against different isoforms of CULT1. Whilst ab73885, ab85994 and ab98273 have been raised against immunogen sequences from CULT1 isoform 1 (SwissProt P39880) and would be expected to react with all CULT1 isoforms, ab115854 and ab54583 have both been raised against an immunogen sequence taken from CULT1 isoform 4 (SwissProt Q13948). These would therefore be expected to recognise isoform 4 and isoform 8 of CULT1 only (expected at around 77 kDa). I'm sorry if this information may not have been made explicit on the datasheet. Unfortunately I have no knowledge of any of the antibodies being produced by the group mentioned. You have not clarified the application or type of sample intended with the antibody but ab73885 would be a good choice if intending to perform Western blotting or ELISA with human CUTL1. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

DISCOUNT CODE: Expiration date: I am very pleased to hear you would like to accept our offer and test ab73885 in IHC on finch. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for IHC on finch tissue and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting Abcam about ab73885 (anti-CUTL1). The anti-CUTL1 is a rabbit polyclonal and so we do not know exactly which epitopes that it recognises on human CUTL1 and unfortunately I do not have anymore information other than it was raised against the N-terminus. Also as the antibody has not been tested on either IHC-Fr or in Finch we cannot guarantee that it will work. I aligned the human and finch sequences and as you said they are very similar, apart from the first 2-30aa and I think it would work. We do not have any sample sizes for you to test with this antibody but we do have a testing discount offer. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab73885 in IHC-Fr in z.finch. I will then send a discount code. This code must be issued before purchasing ab73885 so please wait for my reply before ordering. 2. Purchase ab73885 either by phone, fax, or online (www.abcam.com). 3. Test it in IHC-Fr in z.finch. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab73885 turns out to be unsuitable for IHC-Fr in z.finch, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Application
Western blot
Sample
Human Cell lysate - whole cell (MDA-MB-231)
Loading amount
10000 cells
Specification
MDA-MB-231
Gel Running Conditions
Reduced Denaturing (8%gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Ms. Sabine Topka

Verified customer

Submitted Sep 23 2009

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