Anti-Homer (1b+1c) antibody (ab97593)
Key features and details
- Rabbit polyclonal to Homer (1b+1c)
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Homer (1b+1c) antibody -
Description
Rabbit polyclonal to Homer (1b+1c) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow
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Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 72 and 309 of Human Homer (1b+1c) (NP_004263)
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab97593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 40 kDa. | |
| IHC-P | 1/100 - 1/250. |
Target
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Relevance
Homer is a neuronal immediate early gene that is enriched at excitatory synapses and selectively binds metabotropic glutamate receptors. -
Cellular localization
Cytoplasmic; Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Note=Postsynaptic density of neuronal cells. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum. -
Database links
- Entrez Gene: 9456 Human
- Entrez Gene: 29546 Rat
- Omim: 604798 Human
- SwissProt: Q86YM7 Human
- SwissProt: Q9Z214 Rat
- Unigene: 591761 Human
- Unigene: 37500 Rat
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Alternative names
- Homer 1 antibody
- HOMER antibody
- Homer homolog 1 antibody
see all
Images
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Western blot - Anti-Homer (1b+1c) antibody (ab97593)All lanes : Anti-Homer (1b+1c) antibody (ab97593) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 40 kDa
Gel conc 10% (SDS PAGE) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer (1b+1c) antibody (ab97593)ab97593, at a 1/100 dilution, staining human Homer (1b+1c) in OVCA, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
Protocols
References (2)
ab97593 has been referenced in 2 publications.
- Matosin N et al. Molecular evidence of synaptic pathology in the CA1 region in schizophrenia. NPJ Schizophr 2:16022 (2016). WB . PubMed: 27430010
- Elagabani MN et al. Subunit-selective N-Methyl-d-aspartate (NMDA) Receptor Signaling through Brefeldin A-resistant Arf Guanine Nucleotide Exchange Factors BRAG1 and BRAG2 during Synapse Maturation. J Biol Chem 291:9105-18 (2016). PubMed: 26884337
