Anti-Homer (1b+1c) antibody (ab97593)
- Datasheet
- References (2)
- Protocols
Overview
-
Product name
Anti-Homer (1b+1c) antibody -
Description
Rabbit polyclonal to Homer (1b+1c) -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow
-
Immunogen
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 72 and 309 of Human Homer (1b+1c) (NP_004263)
-
Positive control
- 293T, A431, H1299, HepG2 or Molt-4 cell lysate OVCA tissue
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab97593 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 40 kDa. | |
| IHC-P | 1/100 - 1/250. | |
| IHC-Fr | 1/200. |
Target
-
Relevance
Homer is a neuronal immediate early gene that is enriched at excitatory synapses and selectively binds metabotropic glutamate receptors. -
Cellular localization
Cytoplasmic; Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Note=Postsynaptic density of neuronal cells. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum. -
Database links
- Entrez Gene: 9456 Human
- Entrez Gene: 26556 Mouse
- Entrez Gene: 29546 Rat
- Omim: 604798 Human
- SwissProt: Q86YM7 Human
- SwissProt: Q9Z2Y3 Mouse
- SwissProt: Q9Z214 Rat
- Unigene: 591761 Human
see all -
Alternative names
- Homer 1 antibody
- HOMER antibody
- Homer homolog 1 antibody
see all
Images
-
Western blot - Anti-Homer (1b+1c) antibody (ab97593)All lanes : Anti-Homer (1b+1c) antibody (ab97593) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 40 kDa
Gel conc 10% (SDS PAGE) -
Immunohistochemistry (Frozen sections) - Anti-Homer (1b+1c) antibody (ab97593)Image courtesy of an anonymous Abreview.ab97593 staining Homer (1b+1c) in mouse brain tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, blocked with 1% BSA for 60 minutes at 25°C. Samples were incubated with primary antibody (1/200 in 1% BSA + 0.1% Tx-PBS) for 16 hours at 25°C. An Alexa Flour® 488-conjugated goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer (1b+1c) antibody (ab97593)ab97593, at a 1/100 dilution, staining human Homer (1b+1c) in OVCA, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
References
This product has been referenced in:
- Matosin N et al. Molecular evidence of synaptic pathology in the CA1 region in schizophrenia. NPJ Schizophr 2:16022 (2016). WB . Read more (PubMed: 27430010) »
- Elagabani MN et al. Subunit-selective N-Methyl-d-aspartate (NMDA) Receptor Signaling through Brefeldin A-resistant Arf Guanine Nucleotide Exchange Factors BRAG1 and BRAG2 during Synapse Maturation. J Biol Chem 291:9105-18 (2016). Read more (PubMed: 26884337) »
