Anti-Homer1 antibody (ab211415)

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Overview

  • Product name

    Anti-Homer1 antibody
    See all Homer1 primary antibodies

  • Description

    Rabbit polyclonal to Homer1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Homer1 aa 72-339. The exact sequence is proprietary. NCBI Accession No. NP_004263.1.
    Sequence:

    QKFGQWADS RANTVYGLGF SSEHHLSKFA EKFQEFKEAA RLAKEKSQEK MELTSTPSQE SAGGDLQSPL TPESINGTDD ERTPDVTQNS EPRAEPTQNA LPFSHSSAIS KHWEAELATL KGNNAKLTAA LLESTANVKQ WKQQLAAYQE EAERLHKRVT ELECVSSQAN AVHTHKTELN QTIQELEETL KLKEEEIERL KQEIDNAREL QEQRDSLTQK LQEVEIRNKD LEGQLSDLEQ RLEKSQNEQE AFRNNLKTLL EILDGKIFE


    Database link: Q86YM7

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • IMR32 whole cell lysate/extract; mouse and rat brain lysates/extracts; Human brain tissue; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab211415 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/20000. Predicted molecular weight: 40 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development.

  • Sequence similarities

    Belongs to the Homer family.
    Contains 1 WH1 domain.

  • Domain

    The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3.

  • Cellular localization

    Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • HOME1_HUMAN antibody
    • Homer antibody
    • HOMER 1A antibody
    • HOMER 1B antibody
    • HOMER 1C antibody
    • Homer homolog 1 (Drosophila) antibody
    • homer homolog 1 antibody
    • Homer protein homolog 1 antibody
    • homer scaffolding protein 1 antibody
    • Homer, Drosophila, homolog of, 1 antibody
    • Homer, neuronal immediate early gene, 1 antibody
    • Homer-1 antibody
    • Homer1 antibody
    • HOMER1A antibody
    • HOMER1B antibody
    • HOMER1C antibody
    • SYN47 antibody
    • Ves 1 antibody
    see all

Images

  • Western blot - Anti-Homer1 antibody (ab211415)
    Western blot - Anti-Homer1 antibody (ab211415)
    Anti-Homer1 antibody (ab211415) at 1/1000 dilution + IMR32 whole cell lysate/extract at 30 µg

    Predicted band size: 40 kDa



    10 % SDS-PAGE

  • Western blot - Anti-Homer1 antibody (ab211415)
    Western blot - Anti-Homer1 antibody (ab211415)
    Anti-Homer1 antibody (ab211415) at 1/500 dilution + rat brain lysate/extract at 50 µg

    Predicted band size: 40 kDa



    10 % SDS-PAGE

  • Western blot - Anti-Homer1 antibody (ab211415)
    Western blot - Anti-Homer1 antibody (ab211415)
    Anti-Homer1 antibody (ab211415) at 1/20000 dilution + mouse brain lysate/extract at 20 µg

    Predicted band size: 40 kDa



    10 % SDS-PAGE

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody (ab211415)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody (ab211415)

    Immunohistochemical analysis of formalin-fixed paraffin-embedded Human brain tissue, labeling Homer1 using ab211415 at 7.5 µg/mL.

  • Immunocytochemistry/ Immunofluorescence - Anti-Homer1 antibody (ab211415)
    Immunocytochemistry/ Immunofluorescence - Anti-Homer1 antibody (ab211415)

    Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Homer1 using ab211415 at a 1/500 dilution (green, above). The image below was costained with Hoechst 33342 (blue).

References

This product has been referenced in:

  • Lum JS  et al. Chronic Adolescent CDPPB Treatment Alters Short-Term, but not Long-Term, Glutamatergic Receptor Expression. Neurochem Res N/A:N/A (2018). Read more (PubMed: 29936568) »
  • Lum JS  et al. A postmortem analysis of NMDA ionotropic and group 1 metabotropic glutamate receptors in the nucleus accumbens in schizophrenia. J Psychiatry Neurosci 43:102-110 (2018). Read more (PubMed: 29481317) »
See all 4 Publications for this product

Publishing research using ab211415? Please let us know so that we can cite the reference in this datasheet.

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