Anti-Homer1 antibody (ab211415)
Key features and details
- Rabbit polyclonal to Homer1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Homer1 antibody
See all Homer1 primary antibodies -
Description
Rabbit polyclonal to Homer1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human Homer1 aa 72-339. The exact sequence is proprietary. NCBI Accession No. NP_004263.1.
Sequence:QKFGQWADS RANTVYGLGF SSEHHLSKFA EKFQEFKEAA RLAKEKSQEK MELTSTPSQE SAGGDLQSPL TPESINGTDD ERTPDVTQNS EPRAEPTQNA LPFSHSSAIS KHWEAELATL KGNNAKLTAA LLESTANVKQ WKQQLAAYQE EAERLHKRVT ELECVSSQAN AVHTHKTELN QTIQELEETL KLKEEEIERL KQEIDNAREL QEQRDSLTQK LQEVEIRNKD LEGQLSDLEQ RLEKSQNEQE AFRNNLKTLL EILDGKIFE
Database link: Q86YM7 -
Positive control
- IMR32 whole cell lysate/extract; mouse and rat brain lysates/extracts; Human brain tissue; HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 1.79% Tris glycine -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab211415 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 7.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/500 - 1/20000. Predicted molecular weight: 40 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. -
Sequence similarities
Belongs to the Homer family.
Contains 1 WH1 domain. -
Domain
The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3. -
Cellular localization
Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 9456 Human
- Entrez Gene: 26556 Mouse
- Entrez Gene: 29546 Rat
- Omim: 604798 Human
- SwissProt: Q86YM7 Human
- SwissProt: Q9Z2Y3 Mouse
- SwissProt: Q9Z214 Rat
- Unigene: 591761 Human
see all -
Alternative names
- HOME1_HUMAN antibody
- Homer antibody
- HOMER 1A antibody
see all
Images
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Western blot - Anti-Homer1 antibody (ab211415)Anti-Homer1 antibody (ab211415) at 1/1000 dilution + IMR32 whole cell lysate/extract at 30 µg
Predicted band size: 40 kDa10 % SDS-PAGE
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Western blot - Anti-Homer1 antibody (ab211415)Anti-Homer1 antibody (ab211415) at 1/500 dilution + rat brain lysate/extract at 50 µg
Predicted band size: 40 kDa10 % SDS-PAGE
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Western blot - Anti-Homer1 antibody (ab211415)Anti-Homer1 antibody (ab211415) at 1/20000 dilution + mouse brain lysate/extract at 20 µg
Predicted band size: 40 kDa10 % SDS-PAGE
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody (ab211415)Immunohistochemical analysis of formalin-fixed paraffin-embedded Human brain tissue, labeling Homer1 using ab211415 at 7.5 µg/mL.
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Immunocytochemistry/ Immunofluorescence - Anti-Homer1 antibody (ab211415)Immunofluorescence analysis of methanol-fixed HeLa cells, labeling Homer1 using ab211415 at a 1/500 dilution (green, above). The image below was costained with Hoechst 33342 (blue).
Protocols
References (5)
ab211415 has been referenced in 5 publications.
- Bandleon S et al. FKBP52 regulates TRPC3-dependent Ca2+ signals and the hypertrophic growth of cardiomyocyte cultures. J Cell Sci 132:N/A (2019). PubMed: 31540954
- Lum JS et al. Chronic Adolescent CDPPB Treatment Alters Short-Term, but not Long-Term, Glutamatergic Receptor Expression. Neurochem Res N/A:N/A (2018). PubMed: 29936568
- Lum JS et al. A postmortem analysis of NMDA ionotropic and group 1 metabotropic glutamate receptors in the nucleus accumbens in schizophrenia. J Psychiatry Neurosci 43:102-110 (2018). PubMed: 29481317
- Lum JS et al. A postmortem analysis of NMDA ionotropic and group 1 metabotropic glutamate receptors in the nucleus accumbens in schizophrenia. J Psychiatry Neurosci 42:170077 (2017). PubMed: 29022871
- Lum JS et al. Neurodevelopmental Expression Profile of Dimeric and Monomeric Group 1 mGluRs: Relevance to Schizophrenia Pathogenesis and Treatment. Sci Rep 6:34391 (2016). PubMed: 27721389
