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  1. Link

    homer1-antibody-epr15309-ab184955.pdf

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RecombinantRabMAb

Recombinant Anti-Homer1 antibody [EPR15309] (ab184955)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (2) Submit a question References (9)

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Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)
  • Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] (ab184955)
  • Immunoprecipitation - Anti-Homer1 antibody [EPR15309] (ab184955)
  • Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)
  • Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)
  • Anti-Homer1 antibody [EPR15309] (ab184955)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15309] to Homer1
  • Suitable for: IP, WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-Homer1 antibody [EPR15309]
    See all Homer1 primary antibodies
  • Description

    Rabbit monoclonal [EPR15309] to Homer1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WB, IHC-P, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HepG2 and human fetal brain lysates. IP: Human fetal brain lysate. IHC-P: Human gliocytoma tissue. IHC-Fr: Rat and mouse cerebrum tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15309
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurotransmission
    • Receptors / Channels
    • GPCR
    • Glutamate Receptors
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-Homer1 antibody [EPR15309] - BSA and Azide free (ab232668)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human Homer1 protein (ab85334)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab184955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP
1/60 - 1/80.
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr (1)
1/250.
Notes
IP
1/60 - 1/80.
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/250.

Target

  • Function

    Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development.
  • Sequence similarities

    Belongs to the Homer family.
    Contains 1 WH1 domain.
  • Domain

    The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3.
  • Cellular localization

    Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum.
  • Target information above from: UniProt accession Q86YM7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 9456 Human
    • Entrez Gene: 26556 Mouse
    • Entrez Gene: 29546 Rat
    • Omim: 604798 Human
    • SwissProt: Q86YM7 Human
    • SwissProt: Q9Z2Y3 Mouse
    • SwissProt: Q9Z214 Rat
    • Unigene: 591761 Human
    • Unigene: 37533 Mouse
    • Unigene: 37500 Rat
    see all
  • Alternative names

    • HOME1_HUMAN antibody
    • Homer antibody
    • HOMER 1A antibody
    • HOMER 1B antibody
    • HOMER 1C antibody
    • Homer homolog 1 (Drosophila) antibody
    • homer homolog 1 antibody
    • Homer protein homolog 1 antibody
    • homer scaffolding protein 1 antibody
    • Homer, Drosophila, homolog of, 1 antibody
    • Homer, neuronal immediate early gene, 1 antibody
    • Homer-1 antibody
    • Homer1 antibody
    • HOMER1A antibody
    • HOMER1B antibody
    • HOMER1C antibody
    • SYN47 antibody
    • Ves 1 antibody
    see all

Images

  • Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)
    Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)
    Anti-Homer1 antibody [EPR15309] (ab184955) at 1/5000 dilution + HepG2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 40 kDa
    Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)
    Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling Homer1 with ab184955 at 1/250 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on rat cerebrum. is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] (ab184955)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] (ab184955)

    Immunohistochemical analysis of paraffin-embedded human gliocytoma labeling Homer with ab184955 at 1/500 dilution and HRP polymer for Rabbit IgG.  Counterstained with hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Homer1 antibody [EPR15309] (ab184955)
    Immunoprecipitation - Anti-Homer1 antibody [EPR15309] (ab184955)

    Immunoprecipitation analysis of human fetal brain labeling Homer with ab184955 at 1/50 dilution and Anti-rabbit IgG (HRP) specific to the non reduced form of IgG at 1/1500 dilution (+) or PBS (-)

  • Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)
    Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)
    Anti-Homer1 antibody [EPR15309] (ab184955) at 1/20000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 40 kDa
    Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)
    Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Homer1 with ab184955 at 1/250 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum. is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Anti-Homer1 antibody [EPR15309] (ab184955)
    Anti-Homer1 antibody [EPR15309] (ab184955)

Protocols

  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (9)

Publishing research using ab184955? Please let us know so that we can cite the reference in this datasheet.

ab184955 has been referenced in 9 publications.

  • Chen F  et al. Structural Plasticity and Molecular Markers in Hippocampus of Male Rats after Acute Stress. Neuroscience 438:100-115 (2020). PubMed: 32407976
  • Cui X  et al. Homer1 is a Potential Biomarker for Prognosis in Human Colorectal Carcinoma, Possibly in Association with G3BP1 Signaling. Cancer Manag Res 12:2899-2909 (2020). PubMed: 32425603
  • Cozzolino F  et al. New label-free methods for protein relative quantification applied to the investigation of an animal model of Huntington Disease. PLoS One 15:e0238037 (2020). PubMed: 32886703
  • Ye F  et al. Electroacupuncture reduces scopolamine-induced amnesia via mediating the miR-210/SIN3A and miR-183/SIN3A signaling pathway. Mol Med 26:107 (2020). PubMed: 33183243
  • Garcia-Mesa Y  et al. Regional Brain Recovery from Acute Synaptic Injury in Simian Immunodeficiency Virus-Infected Rhesus Macaques Associates with Heme Oxygenase Isoform Expression. J Virol 94:N/A (2020). PubMed: 32669339
  • Santa-Marinha L  et al. Phospholipase D1 Ablation Disrupts Mouse Longitudinal Hippocampal Axis Organization and Functioning. Cell Rep 30:4197-4208.e6 (2020). PubMed: 32209478
  • Elfving B  et al. Differential expression of synaptic markers regulated during neurodevelopment in a rat model of schizophrenia-like behavior. Prog Neuropsychopharmacol Biol Psychiatry 95:109669 (2019). PubMed: 31228641
  • Figueiredo CP  et al. Zika virus replicates in adult human brain tissue and impairs synapses and memory in mice. Nat Commun 10:3890 (2019). PubMed: 31488835
  • Zhang Z  et al. METTL3-mediated N6-methyladenosine mRNA modification enhances long-term memory consolidation. Cell Res N/A:N/A (2018). WB ; Mouse . PubMed: 30297870

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Homer1 antibody [EPR15309]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hippocampus)
Gel Running Conditions
Reduced Denaturing (Gel 10%)
Loading amount
1 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 27 2017

Immunohistochemistry (Frozen sections) abreview for Anti-Homer1 antibody [EPR15309]

Good
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse spinal cord)
Permeabilization
Yes - 0.1% PBS Triton X-100
Specification
Mouse spinal cord
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 18 2015

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