RecombinantRabMAb

Recombinant Anti-Homer1 antibody [EPR15309] (ab184955)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15309] to Homer1
  • Suitable for: IP, WB, IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Homer1 antibody [EPR15309]
    See all Homer1 primary antibodies

  • Description

    Rabbit monoclonal [EPR15309] to Homer1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WB, IHC-P, IHC-Frmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Homer1 aa 100-250. The exact sequence is proprietary.
    Database link: Q86YM7

  • Positive control

    • WB: HepG2 and human fetal brain lysates. IP: Human fetal brain lysate. IHC-P: Human gliocytoma tissue. IHC-Fr: Rat and mouse cerebrum tissue.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab184955 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/60 - 1/80.
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr 1/250.

Target

  • Function

    Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development.

  • Sequence similarities

    Belongs to the Homer family.
    Contains 1 WH1 domain.

  • Domain

    The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3.

  • Cellular localization

    Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • HOME1_HUMAN antibody
    • Homer antibody
    • HOMER 1A antibody
    • HOMER 1B antibody
    • HOMER 1C antibody
    • Homer homolog 1 (Drosophila) antibody
    • homer homolog 1 antibody
    • Homer protein homolog 1 antibody
    • homer scaffolding protein 1 antibody
    • Homer, Drosophila, homolog of, 1 antibody
    • Homer, neuronal immediate early gene, 1 antibody
    • Homer-1 antibody
    • Homer1 antibody
    • HOMER1A antibody
    • HOMER1B antibody
    • HOMER1C antibody
    • SYN47 antibody
    • Ves 1 antibody
    see all

Images

  • Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)
    Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)
    Anti-Homer1 antibody [EPR15309] (ab184955) at 1/5000 dilution + HepG2 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 40 kDa
    Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)
    Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling Homer1 with ab184955 at 1/250 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on rat cerebrum. is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] (ab184955)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] (ab184955)

    Immunohistochemical analysis of paraffin-embedded human gliocytoma labeling Homer with ab184955 at 1/500 dilution and HRP polymer for Rabbit IgG.  Counterstained with hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Homer1 antibody [EPR15309] (ab184955)
    Immunoprecipitation - Anti-Homer1 antibody [EPR15309] (ab184955)

    Immunoprecipitation analysis of human fetal brain labeling Homer with ab184955 at 1/50 dilution and Anti-rabbit IgG (HRP) specific to the non reduced form of IgG at 1/1500 dilution (+) or PBS (-)

  • Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)
    Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)
    Anti-Homer1 antibody [EPR15309] (ab184955) at 1/20000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 40 kDa
    Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands.

  • Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)
    Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Homer1 with ab184955 at 1/250 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum. is observed. The nuclear counterstain was DAPI (Blue).

    Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 ug/ml) dilution.

    Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Anti-Homer1 antibody [EPR15309] (ab184955)
    Anti-Homer1 antibody [EPR15309] (ab184955)

References (5)

Publishing research using ab184955? Please let us know so that we can cite the reference in this datasheet.

ab184955 has been referenced in 5 publications.

  • Chen F  et al. Structural Plasticity and Molecular Markers in Hippocampus of Male Rats after Acute Stress. Neuroscience 438:100-115 (2020). PubMed: 32407976
  • Cui X  et al. Homer1 is a Potential Biomarker for Prognosis in Human Colorectal Carcinoma, Possibly in Association with G3BP1 Signaling. Cancer Manag Res 12:2899-2909 (2020). PubMed: 32425603
  • Cozzolino F  et al. New label-free methods for protein relative quantification applied to the investigation of an animal model of Huntington Disease. PLoS One 15:e0238037 (2020). PubMed: 32886703
  • Elfving B  et al. Differential expression of synaptic markers regulated during neurodevelopment in a rat model of schizophrenia-like behavior. Prog Neuropsychopharmacol Biol Psychiatry 95:109669 (2019). PubMed: 31228641
  • Zhang Z  et al. METTL3-mediated N6-methyladenosine mRNA modification enhances long-term memory consolidation. Cell Res N/A:N/A (2018). WB ; Mouse . PubMed: 30297870

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Western blot abreview for Anti-Homer1 antibody [EPR15309]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Hippocampus)
Gel Running Conditions
Reduced Denaturing (Gel 10%)
Loading amount
1 µg
Specification
Hippocampus
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Jun 27 2017

Immunohistochemistry (Frozen sections) abreview for Anti-Homer1 antibody [EPR15309]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Mouse spinal cord)
Permeabilization
Yes - 0.1% PBS Triton X-100
Specification
Mouse spinal cord
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Nov 18 2015

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