Recombinant Anti-Homer1 antibody [EPR15309] (ab184955)
![Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-217642-anti-homer1-antibody-epr15309-western-blot.jpg "Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15309] to Homer1
- Suitable for: IP, WB, IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Homer1 antibody [EPR15309]
See all Homer1 primary antibodies -
Description
Rabbit monoclonal [EPR15309] to Homer1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2 and human fetal brain lysates. IP: Human fetal brain lysate. IHC-P: Human gliocytoma tissue. IHC-Fr: Rat and mouse cerebrum tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15309 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab184955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP |
1/60 - 1/80.
|
|
| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| IHC-Fr | (1) |
1/250.
|
| Notes |
|---|
|
IP
1/60 - 1/80. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 40 kDa. |
|
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IHC-Fr
1/250. |
Target
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Function
Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. -
Sequence similarities
Belongs to the Homer family.
Contains 1 WH1 domain. -
Domain
The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3. -
Cellular localization
Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 9456 Human
- Entrez Gene: 26556 Mouse
- Entrez Gene: 29546 Rat
- Omim: 604798 Human
- SwissProt: Q86YM7 Human
- SwissProt: Q9Z2Y3 Mouse
- SwissProt: Q9Z214 Rat
- Unigene: 591761 Human
see all -
Alternative names
- HOME1_HUMAN antibody
- Homer antibody
- HOMER 1A antibody
see all
Images
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Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)Anti-Homer1 antibody [EPR15309] (ab184955) at 1/5000 dilution + HepG2 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 40 kDa
Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands. -
![Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-7-anti-homer1-antibody-epr15309-immunohistochemistry-frozen-rat-cerebrum8.jpg)
Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cerebrum tissue labeling Homer1 with ab184955 at 1/250 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on rat cerebrum. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 ug/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-217640-anti-homer1-antibody-epr15309-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] (ab184955)Immunohistochemical analysis of paraffin-embedded human gliocytoma labeling Homer with ab184955 at 1/500 dilution and HRP polymer for Rabbit IgG. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-217638-anti-homer1-antibody-epr15309-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Homer1 antibody [EPR15309] (ab184955)Immunoprecipitation analysis of human fetal brain labeling Homer with ab184955 at 1/50 dilution and Anti-rabbit IgG (HRP) specific to the non reduced form of IgG at 1/1500 dilution (+) or PBS (-)
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![Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-217641-anti-homer1-antibody-epr15309-western-blot.jpg)
Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)Anti-Homer1 antibody [EPR15309] (ab184955) at 1/20000 dilution + Human fetal brain lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 40 kDa
Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands. -
![Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-6-anti-homer1-antibody-epr15309-immunohistochemistry-frozen-mouse-cerebrum8.jpg)
Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebrum tissue labeling Homer1 with ab184955 at 1/250 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Positive staining on mouse cerebrum. is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 (2 ug/ml) dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
-
![Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-8-benefits-of-recombinant-antibodies.png)
Anti-Homer1 antibody [EPR15309] (ab184955)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (9)
ab184955 has been referenced in 9 publications.
- Chen F et al. Structural Plasticity and Molecular Markers in Hippocampus of Male Rats after Acute Stress. Neuroscience 438:100-115 (2020). PubMed: 32407976
- Cui X et al. Homer1 is a Potential Biomarker for Prognosis in Human Colorectal Carcinoma, Possibly in Association with G3BP1 Signaling. Cancer Manag Res 12:2899-2909 (2020). PubMed: 32425603
- Cozzolino F et al. New label-free methods for protein relative quantification applied to the investigation of an animal model of Huntington Disease. PLoS One 15:e0238037 (2020). PubMed: 32886703
- Ye F et al. Electroacupuncture reduces scopolamine-induced amnesia via mediating the miR-210/SIN3A and miR-183/SIN3A signaling pathway. Mol Med 26:107 (2020). PubMed: 33183243
- Garcia-Mesa Y et al. Regional Brain Recovery from Acute Synaptic Injury in Simian Immunodeficiency Virus-Infected Rhesus Macaques Associates with Heme Oxygenase Isoform Expression. J Virol 94:N/A (2020). PubMed: 32669339
- Santa-Marinha L et al. Phospholipase D1 Ablation Disrupts Mouse Longitudinal Hippocampal Axis Organization and Functioning. Cell Rep 30:4197-4208.e6 (2020). PubMed: 32209478
- Elfving B et al. Differential expression of synaptic markers regulated during neurodevelopment in a rat model of schizophrenia-like behavior. Prog Neuropsychopharmacol Biol Psychiatry 95:109669 (2019). PubMed: 31228641
- Figueiredo CP et al. Zika virus replicates in adult human brain tissue and impairs synapses and memory in mice. Nat Commun 10:3890 (2019). PubMed: 31488835
- Zhang Z et al. METTL3-mediated N6-methyladenosine mRNA modification enhances long-term memory consolidation. Cell Res N/A:N/A (2018). WB ; Mouse . PubMed: 30297870