Recombinant Anti-Homer1 antibody [EPR15309] (ab184955)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Homer1 antibody [EPR15309]
See all Homer1 primary antibodies -
Description
Rabbit monoclonal [EPR15309] to Homer1 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WB, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human Homer1 aa 100-250. The exact sequence is proprietary.
Database link: Q86YM7 -
Positive control
- HepG2, human fetal brain lysates, human gliocytoma tissue,
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15309 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab184955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/60 - 1/80. | |
| WB | 1/1000 - 1/10000. Predicted molecular weight: 40 kDa. | |
| IHC-P | 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| IHC-Fr | 1/500. |
Target
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Function
Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development. -
Sequence similarities
Belongs to the Homer family.
Contains 1 WH1 domain. -
Domain
The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3. -
Cellular localization
Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 9456 Human
- Entrez Gene: 26556 Mouse
- Entrez Gene: 29546 Rat
- Omim: 604798 Human
- SwissProt: Q86YM7 Human
- SwissProt: Q9Z2Y3 Mouse
- SwissProt: Q9Z214 Rat
- Unigene: 591761 Human
see all -
Alternative names
- HOME1_HUMAN antibody
- Homer antibody
- HOMER 1A antibody
see all
Images
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![Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-217642-anti-homer1-antibody-epr15309-western-blot.jpg)
Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)Anti-Homer1 antibody [EPR15309] (ab184955) at 1/5000 dilution + HepG2 cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 40 kDa
Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-217640-anti-homer1-antibody-epr15309-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Homer1 antibody [EPR15309] (ab184955)Immunohistochemical analysis of paraffin-embedded human gliocytoma labeling Homer with ab184955 at 1/500 dilution and HRP polymer for Rabbit IgG. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
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![Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-255286-anti-homer1-antibody-epr15309-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-Homer1 antibody [EPR15309] (ab184955)This image is courtesy of an anonymous Abreviewab184955 staining Homer1 in mouse spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% PBS Triton X-100 and blocked with 1% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (0.1% PBS Triton X-100 + 1% BSA) for 16 hours at 4°C. An Alexa Fluor® 488 conjugated goat anti-rabbit IgG (H+L) (1/1000) was used as the secondary antibody.
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![Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-217641-anti-homer1-antibody-epr15309-western-blot.jpg)
Western blot - Anti-Homer1 antibody [EPR15309] (ab184955)Anti-Homer1 antibody [EPR15309] (ab184955) at 1/20000 dilution + Human fetal brain lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 40 kDa
Additional bands at: 46 kDa. We are unsure as to the identity of these extra bands. -
![Immunoprecipitation - Anti-Homer1 antibody [EPR15309] (ab184955)](https://www.abcam.com/ps/products/184/ab184955/Images/ab184955-217638-anti-homer1-antibody-epr15309-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Homer1 antibody [EPR15309] (ab184955)Immunoprecipitation analysis of human fetal brain labeling Homer with ab184955 at 1/50 dilution and Anti-rabbit IgG (HRP) specific to the non reduced form of IgG at 1/1500 dilution (+) or PBS (-)
Datasheets and documents
References
This product has been referenced in:
- Zhang Z et al. METTL3-mediated N6-methyladenosine mRNA modification enhances long-term memory consolidation. Cell Res N/A:N/A (2018). WB ; Mouse . Read more (PubMed: 30297870) »
