Postsynaptic density scaffolding protein. Binds and cross-links cytoplasmic regions of GRM1, GRM5, ITPR1, DNM3, RYR1, RYR2, SHANK1 and SHANK3. By physically linking GRM1 and GRM5 with ER-associated ITPR1 receptors, it aids the coupling of surface receptors to intracellular calcium release. May also couple GRM1 to PI3 kinase through its interaction with AGAP2. Isoform 1 regulates the trafficking and surface expression of GRM5. Isoform 3 acts as a natural dominant negative, in dynamic competition with constitutively expressed isoform 1 to regulate synaptic metabotropic glutamate function. Isoform 3, may be involved in the structural changes that occur at synapses during long-lasting neuronal plasticity and development.
Belongs to the Homer family. Contains 1 WH1 domain.
The WH1 domain interacts with the PPXXF motif in GRM1, GRM5, RYR1, RYR2, ITPR1, SHANK 1 and SHANK3.
Cytoplasm. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Isoform 1 inhibits surface expression of GRM5 causing it to be retained in the endoplasmic reticulum.
Immunohistochemical analysis of paraffin-embedded human gliocytoma labeling Homer with ab184955 at 1/500 dilution and HRP polymer for Rabbit IgG. Counterstained with hematoxylin.
Immunohistochemistry (Frozen sections) - Anti-Homer antibody [EPR15309] (ab184955)This image is courtesy of an anonymous Abreview
ab184955 staining Homer1 in mouse spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% PBS Triton X-100 and blocked with 1% BSA for 1 hour at 21°C. Samples were incubated with primary antibody (0.1% PBS Triton X-100 + 1% BSA) for 16 hours at 4°C. An Alexa Fluor® 488 conjugated goat anti-rabbit IgG (H+L) (1/1000) was used as the secondary antibody.