Overview

  • Product name
    Anti-Homocysteine antibody
    See all Homocysteine primary antibodies
  • Description
    Rabbit polyclonal to Homocysteine
  • Host species
    Rabbit
  • Specificity
    Compound Crossreactivity Ratio Homocysteine G BSA 1 Cysteine G BSA 1/423 Homocysteic acid G BSA 1/100,000
  • Tested applications
    Suitable for: IHC-P, IP, WB, Dot blot, ELISA, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Mammals
  • Immunogen

    Other Immunogen Type corresponding to Homocysteine conjugated to bovine serum albumin (Glutaraldehyde).

Properties

Applications

Our Abpromise guarantee covers the use of ab15154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration.
Dot blot 1/10000.
ELISA Use at an assay dependent concentration.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22419888

Target

  • Relevance
    Homocysteine is a chemical compound with the formula HSCH2CH2CH(NH2)CO2H. It is a homologue of the naturally-occurring amino acid cysteine, differing in that its side-chain contains an additional methylene (-CH2-) group before the thiol (-SH) group. Alternatively, Homocysteine can be derived from methionine by removing the latter's terminal C methyl group. Elevations of Homocysteine also occur in the rare hereditary disease homocystinuria and in methylene-tetrahydrofolate-reductase deficiency. The latter is quite common and usually goes unnoticed, although there are reports that thrombosis and cardiovascular disease occurs more often in people with elevated Homocysteine.
  • Alternative names
    • (2S) 2 amino 4 sulfanyl butanoic acid antibody

References

This product has been referenced in:
See all 6 Publications for this product

Customer reviews and Q&As

1-10 of 11 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 3% hydrogen peroxide
Sample
Mouse Tissue sections (eye)
Specification
eye
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 18 2013

Answer

This product is now published with catalogue number ab135231. Please order the product directly from website.

I hope this information will be helpful. Should you have any other inquiry please do not hesitate to contact me.

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Answer

Thank you for your email.

I have forwarded your request to datasheet team. I will let you know the catalogue number for ordering soon.

Many thanks for having patience!

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Application
Immunoprecipitation
Sample
Rat Tissue lysate - whole (hypocamp rat cell)
Total protein in input
600 µg
Specification
hypocamp rat cell
Immuno-precipitation step
Protein A/G

Abcam user community

Verified customer

Submitted Jun 04 2012

Question
Answer

Thank you for contacting us.

I can confirm that we can provide the peptide you have requested. The size is 1mg and the price would be ˜365£ for 1 vial. Could you please let me know if you are interested in buying this? I will then be asking the datasheet team for publishing this product.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Application
Western blot
Sample
Rat Tissue lysate - whole (hypocamp cell)
Loading amount
20 µg
Specification
hypocamp cell
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 29 2012

Answer

Thank you very much for your interest in ab15154.

To our knowledge,ab15154 has not been tested in WB and we do not have any data this antibody works in WB yet.

Therefore, I can offer a discount off a future purchase if you buyab15154 now, test it inWB and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab15154 in WB. I will then send a discount code. This code must be issued before purchasing ab15154 so please wait for my reply before ordering.

2. Purchase ab15154 either by phone, fax, or online (www.abcam.com).

3. Test it in WB.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab15154 turns out to be unsuitable for WB, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Question
Answer

Thank you for your phone call.

Below please find the information on how the testing discount program works. I have selected ab15154 as an example.

Thank you very much for your interest in ab15154.

To our knowledge, ab15154 has not been tested in sandwich ELISA. Therefore, I can offer a discount off a future purchase if you buy ab15154 now, test it in sandwich ELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab15154 in sandwich ELISA. I will then send a discount code. This code must be issued before purchasing ab15154 so please wait for my reply before ordering.

2. Purchase ab15154 either by phone, fax, or online (www.abcam.com).

3. Test it in sandwich ELISA.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab15154 turns out to be unsuitable for sandwich ELISA, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for getting back to me. In response to your customer; I am sorry for the confusion. I do not know where the peptide sequence that your customer cites was sourced. I can confirm that this antibody was raised against homocysteine-glutaraldehyde-BSA. I can also confirm that this antibody does not recognize Homocysteine alone and only recognizes homocysteine in the context of BSA-homocysteine. This antibody is sourced from an external originator. Unfortunately we do not have details of the testing of this antibody against the uncoupled form of BSA. I am sorry that I cannot be of more assistance.

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1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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