Overview

  • Product name
    Anti-Homocysteine antibody
    See all Homocysteine primary antibodies
  • Description
    Rabbit polyclonal to Homocysteine
  • Host species
    Rabbit
  • Specificity
    Using conjugated Homocysteine-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds:Compound : Cross-reactivity ratio(a) Cysteine-G-BSA: 1/423 Homocysteic acid-G-BSA: 1/>100000 (a) Homocysteine-G-BSA concentration/unconjugated or conjugated amino acids concentration at half displacement G = Glutaraldehyde, BSA = Bovine Serum Albumin
  • Tested applications
    Suitable for: ELISAmore details
  • Immunogen

    Chemical/ Small Molecule by a Glutaraldehyde linker.

Properties

Applications

Our Abpromise guarantee covers the use of ab6482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/2000 - 1/10000.

Dilute in PBS.

Target

  • Relevance
    Homocysteine is a chemical compound with the formula HSCH2CH2CH(NH2)CO2H. It is a homologue of the naturally-occurring amino acid cysteine, differing in that its side-chain contains an additional methylene (-CH2-) group before the thiol (-SH) group. Alternatively, Homocysteine can be derived from methionine by removing the latter's terminal C methyl group. Elevations of Homocysteine also occur in the rare hereditary disease homocystinuria and in methylene-tetrahydrofolate-reductase deficiency. The latter is quite common and usually goes unnoticed, although there are reports that thrombosis and cardiovascular disease occurs more often in people with elevated Homocysteine.
  • Alternative names
    • (2S) 2 amino 4 sulfanyl butanoic acid antibody

References

This product has been referenced in:
  • Ganapathy PS  et al. Endogenous elevation of homocysteine induces retinal neuron death in the cystathionine-beta-synthase mutant mouse. Invest Ophthalmol Vis Sci 50:4460-70 (2009). Read more (PubMed: 19357353) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Question
Answer

Thank you for your phone call.

Below please find the information on how the testing discount program works. I have selected ab15154 as an example.

Thank you very much for your interest in ab15154.

To our knowledge, ab15154 has not been tested in sandwich ELISA. Therefore, I can offer a discount off a future purchase if you buy ab15154 now, test it in sandwich ELISA and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab15154 in sandwich ELISA. I will then send a discount code. This code must be issued before purchasing ab15154 so please wait for my reply before ordering.

2. Purchase ab15154 either by phone, fax, or online (www.abcam.com).

3. Test it in sandwich ELISA.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab15154 turns out to be unsuitable for sandwich ELISA, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

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Answer

The originator of ab6482 was only able to tell me that when ab6482 was tested in ELISA, conjugated homocysteine was used and not free homocysteine. Unfortunately I cannot provide you with any more information at this time.

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Answer

Thank you for your enquiry. All the information that we have at this time is located on the online datasheet. The antibody was tested in ELISA using conjugated Homocysteine-Glutaraldehyde-Protein.

Read More

Answer

1. We estimate the molecular weight of this antibody to be between 150-160 kDa and the concentration to be around 1mg/ml.
2. The epitope has not been mapped
3. We have not tested stability in ascorbic acid
We suggest that the antibody is stored according to the conditions on the datasheet

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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