Overview

  • Product name

  • Description

    Rabbit polyclonal to HOOK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HOOK1 aa 150-200. The exact sequence is proprietary.
    Database link: Q9UJC3

  • Positive control

    • IP: HOOK1 IP in HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab241433 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-10 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Function

      Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex).
    • Sequence similarities

      Belongs to the hook family.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cytoplasm. Localizes to the spermatid manchette during spermiogenesis but is not present in mature spermatozoa (By similarity). Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules.
    • Information by UniProt
    • Database links

    • Alternative names

      • A930033L17Rik antibody
      • Abnormal spermatozoon head shape antibody
      • azh antibody
      • h-hook1 antibody
      • hHK1 antibody
      • HK1 antibody
      • HOOK 1 antibody
      • Hook homolog 1 (Drosophila) antibody
      • Hook1 antibody
      • HOOK1_HUMAN antibody
      • MGC10642 antibody
      • OTTHUMP00000010548 antibody
      • OTTMUSP00000008480 antibody
      • Protein Hook homolog 1 antibody
      • RP23-80B16.4 antibody
      see all

    Images

    • HOOK1 was immunoprecipitated from HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
      ab241433 used for IP at 6 µg per reaction. For WB 0.4 µg/ml.

      Lane 1: ab241433 IP in HEK-293T whole cell lysate.
      Lane 2: Control IgG.

      Chemiluminescence detection: 30 seconds.

    References

    ab241433 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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