Overview

  • Product name

    Anti-HOOK1 antibody [EPR10102]
    See all HOOK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10102] to HOOK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human HOOK1 (Q9UJC3)

  • Positive control

    • 293T or MCF-7 lysate, Human testis tissue or Caco-2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex).
    • Sequence similarities

      Belongs to the hook family.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cytoplasm. Localizes to the spermatid manchette during spermiogenesis but is not present in mature spermatozoa (By similarity). Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules.
    • Information by UniProt
    • Database links

    • Alternative names

      • A930033L17Rik antibody
      • Abnormal spermatozoon head shape antibody
      • azh antibody
      • h-hook1 antibody
      • hHK1 antibody
      • HK1 antibody
      • HOOK 1 antibody
      • Hook homolog 1 (Drosophila) antibody
      • Hook1 antibody
      • HOOK1_HUMAN antibody
      • MGC10642 antibody
      • OTTHUMP00000010548 antibody
      • OTTMUSP00000008480 antibody
      • Protein Hook homolog 1 antibody
      • RP23-80B16.4 antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin embedded Human testis tissue labeling HOOK1 with ab151756 at a 1/100 dilution.
    • All lanes : Anti-HOOK1 antibody [EPR10102] (ab151756) at 1/1000 dilution

      Lane 1 : 293T cell lysate
      Lane 2 : MCF-7 cell lysate
      Lane 3 : Caco-2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 85 kDa

    • Immunofluorescence analysis of Caco-2 cells labeling HOOK1 with ab151756 at a 1/100 dilution.
    • Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab151756 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab151756 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab151756 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab151756 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab151756 showing +ve staining.

    References

    This product has been referenced in:

    • Dwivedi D  et al. The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis. J Cell Biol 218:871-894 (2019). Read more (PubMed: 30674580) »
    • Cao J  et al. Clinicopathological and prognostic significance of SHP2 and Hook1 expression in patients with thyroid carcinoma. Hum Pathol N/A:N/A (2018). Read more (PubMed: 29953894) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (293T cells)
    Gel Running Conditions
    Reduced Denaturing (4-12% Bis-Tris gel (20 well))
    Loading amount
    20 µg
    Specification
    293T cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Aug 14 2018

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