Recombinant Anti-HOOK1 antibody [EPR10102] (ab151756)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10102] to HOOK1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
Overview
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Product name
Anti-HOOK1 antibody [EPR10102]
See all HOOK1 primary antibodies -
Description
Rabbit monoclonal [EPR10102] to HOOK1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to residues in Human HOOK1 (Q9UJC3)
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Positive control
- 293T or MCF-7 lysate, Human testis tissue or Caco-2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR10102 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab151756 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | 1/1000 - 1/10000. Predicted molecular weight: 85 kDa. | |
IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended. | |
ICC/IF | 1/100 - 1/250. |
Target
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Function
Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). -
Sequence similarities
Belongs to the hook family. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm. Localizes to the spermatid manchette during spermiogenesis but is not present in mature spermatozoa (By similarity). Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules. - Information by UniProt
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Database links
- Entrez Gene: 51361 Human
- Omim: 607820 Human
- SwissProt: Q9UJC3 Human
- Unigene: 378836 Human
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Alternative names
- A930033L17Rik antibody
- Abnormal spermatozoon head shape antibody
- azh antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)
Immunohistochemical analysis of paraffin embedded Human testis tissue labeling HOOK1 with ab151756 at a 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-HOOK1 antibody [EPR10102] (ab151756) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 85 kDa -
Immunofluorescence analysis of Caco-2 cells labeling HOOK1 with ab151756 at a 1/100 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)
Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab151756 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)
Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab151756 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)
Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab151756 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)
Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab151756 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)
Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab151756 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References (2)
ab151756 has been referenced in 2 publications.
- Dwivedi D et al. The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis. J Cell Biol 218:871-894 (2019). PubMed: 30674580
- Cao J et al. Clinicopathological and prognostic significance of SHP2 and Hook1 expression in patients with thyroid carcinoma. Hum Pathol N/A:N/A (2018). PubMed: 29953894