Name: Anti-HOOK1 antibody [EPR10102]
Brand: RabMAb
SKU: ab151756
Rating: 5 (1 reviews)

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Product name

Anti-HOOK1 antibody [EPR10102]
See all HOOK1 primary antibodies
Description

Rabbit monoclonal [EPR10102] to HOOK1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide corresponding to residues in Human HOOK1 (Q9UJC3)
Positive control
- 293T or MCF-7 lysate, Human testis tissue or Caco-2 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR10102
Isotype

IgG
Research areas

Alternative Versions
- Anti-HOOK1 antibody [EPR10102] - BSA and Azide free (ab249067)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- MCF7 whole cell lysate (ab3871)
- 293T whole cell lysate (ab95494)

Our Abpromise guarantee covers the use of ab151756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended.
ICC/IF		1/100 - 1/250.

Application notes

Is unsuitable for Flow Cyt or IP.

Function

Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex).
Sequence similarities

Belongs to the hook family.
Cellular localization

Cytoplasm > cytoskeleton. Cytoplasm. Localizes to the spermatid manchette during spermiogenesis but is not present in mature spermatozoa (By similarity). Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules.
Target information above from: UniProt accession Q9UJC3 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 51361 Human
- Omim: 607820 Human
- SwissProt: Q9UJC3 Human
- Unigene: 378836 Human
Alternative names
- A930033L17Rik antibody
- Abnormal spermatozoon head shape antibody
- azh antibody
- h-hook1 antibody
- hHK1 antibody
- HK1 antibody
- HOOK 1 antibody
- Hook homolog 1 (Drosophila) antibody
- Hook1 antibody
- HOOK1_HUMAN antibody
- MGC10642 antibody
- OTTHUMP00000010548 antibody
- OTTMUSP00000008480 antibody
- Protein Hook homolog 1 antibody
- RP23-80B16.4 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

Immunohistochemical analysis of paraffin embedded Human testis tissue labeling HOOK1 with ab151756 at a 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-HOOK1 antibody [EPR10102] (ab151756)

All lanes : Anti-HOOK1 antibody [EPR10102] (ab151756) at 1/1000 dilution

Lane 1 : 293T cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : Caco-2 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 85 kDa
Immunocytochemistry/ Immunofluorescence - Anti-HOOK1 antibody [EPR10102] (ab151756)

Immunofluorescence analysis of Caco-2 cells labeling HOOK1 with ab151756 at a 1/100 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab151756 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab151756 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab151756 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab151756 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab151756 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Datasheet

This product has been referenced in:

Dwivedi D et al. The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis. J Cell Biol 218:871-894 (2019). Read more (PubMed: 30674580) »
Cao J et al. Clinicopathological and prognostic significance of SHP2 and Hook1 expression in patients with thyroid carcinoma. Hum Pathol N/A:N/A (2018). Read more (PubMed: 29953894) »

See all 2 Publications for this product

Publishing research using ab151756? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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Application

Western blot

Sample

Human Cell lysate - whole cell (293T cells)

Gel Running Conditions

Reduced Denaturing (4-12% Bis-Tris gel (20 well))

Loading amount

20 µg

Specification

293T cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Aug 14 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Recombinant Anti-HOOK1 antibody [EPR10102] (ab151756)

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Applications

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As

Western blot abreview for Anti-HOOK1 antibody [EPR10102]