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  1. Link

    hook1-antibody-epr10102-ab151756.pdf

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Signal Transduction Cytoskeleton / ECM Cytoskeleton Microtubules MT Associated Proteins MAP
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RecombinantRabMAb

Recombinant Anti-HOOK1 antibody [EPR10102] (ab151756)

  • Datasheet
Reviews (1) Submit a question References (2)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)
  • Western blot - Anti-HOOK1 antibody [EPR10102] (ab151756)
  • Immunocytochemistry/ Immunofluorescence - Anti-HOOK1 antibody [EPR10102] (ab151756)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)
  • Anti-HOOK1 antibody [EPR10102] (ab151756)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10102] to HOOK1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-HOOK1 antibody [EPR10102]
    See all HOOK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10102] to HOOK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human HOOK1 (Q9UJC3)

  • Positive control

    • 293T or MCF-7 lysate, Human testis tissue or Caco-2 cells.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10102
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Microtubules
    • MT Associated Proteins
    • MAP
    • Signal Transduction
    • Protein Trafficking
    • Organelle Proteins
    • Cell Biology
    • Cell Cycle
    • Cell differentiation
    • Cancer
    • Cell cycle
    • Cell differentiation
    • Stem Cells
    • Germline Stem Cells
    • Spermatogonial Stem Cells

Associated products

  • Alternative Versions

    • Anti-HOOK1 antibody [EPR10102] - BSA and Azide free (ab249067)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

Our Abpromise guarantee covers the use of ab151756 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Use of an HRP/AP polymerized secondary antibody recommended.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex).
    • Sequence similarities

      Belongs to the hook family.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cytoplasm. Localizes to the spermatid manchette during spermiogenesis but is not present in mature spermatozoa (By similarity). Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules.
    • Target information above from: UniProt accession Q9UJC3 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 51361 Human
      • Omim: 607820 Human
      • SwissProt: Q9UJC3 Human
      • Unigene: 378836 Human
      • Alternative names

        • A930033L17Rik antibody
        • Abnormal spermatozoon head shape antibody
        • azh antibody
        • h-hook1 antibody
        • hHK1 antibody
        • HK1 antibody
        • HOOK 1 antibody
        • Hook homolog 1 (Drosophila) antibody
        • Hook1 antibody
        • HOOK1_HUMAN antibody
        • MGC10642 antibody
        • OTTHUMP00000010548 antibody
        • OTTMUSP00000008480 antibody
        • Protein Hook homolog 1 antibody
        • RP23-80B16.4 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

        Immunohistochemical analysis of paraffin embedded Human testis tissue labeling HOOK1 with ab151756 at a 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Western blot - Anti-HOOK1 antibody [EPR10102] (ab151756)
        Western blot - Anti-HOOK1 antibody [EPR10102] (ab151756)
        All lanes : Anti-HOOK1 antibody [EPR10102] (ab151756) at 1/1000 dilution

        Lane 1 : 293T cell lysate
        Lane 2 : MCF-7 cell lysate
        Lane 3 : Caco-2 cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size: 85 kDa

      • Immunocytochemistry/ Immunofluorescence - Anti-HOOK1 antibody [EPR10102] (ab151756)
        Immunocytochemistry/ Immunofluorescence - Anti-HOOK1 antibody [EPR10102] (ab151756)
        Immunofluorescence analysis of Caco-2 cells labeling HOOK1 with ab151756 at a 1/100 dilution.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

        Immunohistochemical analysis of paraffin embedded Human normal brain tissue using ab151756 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

        Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab151756 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

        Immunohistochemical analysis of paraffin embedded Human ovarian carcinoma tissue using ab151756 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

        Immunohistochemical analysis of paraffin embedded Human thyroid gland carcinoma tissue using ab151756 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HOOK1 antibody [EPR10102] (ab151756)

        Immunohistochemical analysis of paraffin embedded Human normal uterus tissue using ab151756 showing +ve staining.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Anti-HOOK1 antibody [EPR10102] (ab151756)
        Anti-HOOK1 antibody [EPR10102] (ab151756)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (2)

      Publishing research using ab151756? Please let us know so that we can cite the reference in this datasheet.

      ab151756 has been referenced in 2 publications.

      • Dwivedi D  et al. The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis. J Cell Biol 218:871-894 (2019). PubMed: 30674580
      • Cao J  et al. Clinicopathological and prognostic significance of SHP2 and Hook1 expression in patients with thyroid carcinoma. Hum Pathol N/A:N/A (2018). PubMed: 29953894

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Western blot abreview for Anti-HOOK1 antibody [EPR10102]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (293T cells)
      Gel Running Conditions
      Reduced Denaturing (4-12% Bis-Tris gel (20 well))
      Loading amount
      20 µg
      Specification
      293T cells
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Aug 14 2018

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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