Western blot abreview for Anti-HOOK1 antibody [EPR10102]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (293T cells)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris gel (20 well))
Loading amount
20 µg
Specification
293T cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% milk
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL (western lightning)
Negative control
293T HOOK1 knockout cell line generated by CRISPR
Exposure
30 second(s)
Bands
Specific: between 80-90 kDa
Positive control
Native 293T extract (this cell type expresses HOOK1)

Additional data

Additional Notes
PVDF membrane was used for transfer/Tris-Glycine wet transfer Ponceau S staining revealed uniform loading across the lanes. Lanes are as follows: 1.) 293T 2.) 293T HOOK1Het 3.) 293T HOOK1 KO 4.) 293T CRISPR control (empty gRNA vector)

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Submitted Aug 14 2018

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