Overview

  • Product name

    Anti-HOOK1 antibody [EPR10102] - BSA and Azide free
    See all HOOK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10102] to HOOK1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HOOK1. The exact sequence is proprietary.
    Database link: Q9UJC3

  • General notes

    Ab249067 is the carrier-free version of ab151756. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab249067 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab249067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 85 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Use of a HRP/AP polymerized secondary antibody is recommended.

 

  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex).
    • Sequence similarities

      Belongs to the hook family.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cytoplasm. Localizes to the spermatid manchette during spermiogenesis but is not present in mature spermatozoa (By similarity). Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules.
    • Information by UniProt
    • Database links

    • Alternative names

      • A930033L17Rik antibody
      • Abnormal spermatozoon head shape antibody
      • azh antibody
      • h-hook1 antibody
      • hHK1 antibody
      • HK1 antibody
      • HOOK 1 antibody
      • Hook homolog 1 (Drosophila) antibody
      • Hook1 antibody
      • HOOK1_HUMAN antibody
      • MGC10642 antibody
      • OTTHUMP00000010548 antibody
      • OTTMUSP00000008480 antibody
      • Protein Hook homolog 1 antibody
      • RP23-80B16.4 antibody
      see all

    References

    ab249067 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab249067.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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