Overview

  • Product name

    Anti-HOOK1 antibody [EPR10103(B)]
    See all HOOK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10103(B)] to HOOK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human HOOK1 aa 700-800 (C terminal). The exact sequence is proprietary.

  • Positive control

    • A673, MCF-7 and 293T cell lysates.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab150397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 85 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • Target

    • Function

      Required for spermatid differentiation. Probably involved in the positioning of the microtubules of the manchette and the flagellum in relation to the membrane skeleton (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex).
    • Sequence similarities

      Belongs to the hook family.
    • Cellular localization

      Cytoplasm > cytoskeleton. Cytoplasm. Localizes to the spermatid manchette during spermiogenesis but is not present in mature spermatozoa (By similarity). Localizes to punctate cytoplasmic foci which do not appear to overlap with early or late endosomes, the endoplasmic reticulum, the Golgi complex, multivesicular bodies (MVBs), lysosomes, or mitochondria. Often found in close association with microtubules.
    • Information by UniProt
    • Database links

    • Alternative names

      • A930033L17Rik antibody
      • Abnormal spermatozoon head shape antibody
      • azh antibody
      • h-hook1 antibody
      • hHK1 antibody
      • HK1 antibody
      • HOOK 1 antibody
      • Hook homolog 1 (Drosophila) antibody
      • Hook1 antibody
      • HOOK1_HUMAN antibody
      • MGC10642 antibody
      • OTTHUMP00000010548 antibody
      • OTTMUSP00000008480 antibody
      • Protein Hook homolog 1 antibody
      • RP23-80B16.4 antibody
      see all

    Images

    • All lanes : Anti-HOOK1 antibody [EPR10103(B)] (ab150397) at 1/1000 dilution

      Lane 1 : A673 cell lysate
      Lane 2 : MCF7 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 85 kDa

    References

    This product has been referenced in:

    • Do M  et al. Quantitative proteomic analysis of pancreatic cyst fluid proteins associated with malignancy in intraductal papillary mucinous neoplasms. Clin Proteomics 15:17 (2018). Read more (PubMed: 29713252) »
    • Olenick MA  et al. Hook Adaptors Induce Unidirectional Processive Motility by Enhancing the Dynein-Dynactin Interaction. J Biol Chem 291:18239-51 (2016). Read more (PubMed: 27365401) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab150397.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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