Anti-HOOK2 antibody (ab154109)
Key features and details
- Rabbit polyclonal to HOOK2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-HOOK2 antibody
See all HOOK2 primary antibodies -
Description
Rabbit polyclonal to HOOK2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 197-380 of Human HOOK2 (UniProt ID: Q96ED9).
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Positive control
- WB: HepG2, HCT116 and mouse liver whole cell lysates. IHC-P: Human MCF7 xenograft; Mouse duodenum and testis tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154109 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 83 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 83 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Relevance
HOOK2 is a member of the Hook protein family. Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. HOOK2 localizes to the centrosome through all phases of the cell cycle. Interference with HOOK2 function results in the loss of the radial organization of microtubules suggesting that HOOK2 contributes to the establishment and maintenance of centrosomal structure and function. HOOK2 may contribute to the establishment and maintenance of the pericentrosomal localization of aggresomes by promoting the microtubule-based delivery of protein aggregates to pericentriolar aggresomes. -
Cellular localization
Cytoplasmic. Note=Associates with discrete punctate structure that correspond to neither early or late endosomes, lysosomes, multivesicular bodies (MVBs), Golgi complex, endoplasmic reticulum, nor mitochondria. -
Database links
- Entrez Gene: 526516 Cow
- Entrez Gene: 29911 Human
- Entrez Gene: 170833 Mouse
- Entrez Gene: 304669 Rat
- Omim: 607824 Human
- SwissProt: Q96ED9 Human
- SwissProt: Q7TMK6 Mouse
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Alternative names
- A630054I03Rik antibody
- FLJ26218 antibody
- h hook2 antibody
see all
Images
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Paraffin-embedded mouse duodenum tissue stained for HOOK2 using ab154109 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse testis tissue stained for HOOK2 using ab154109 at 1/500 dilution in immunohistochemical analysis.
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PFA-fixed, Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HOOK2 (green) using ab154109 at 1/500 dilution in ICC/IF.
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Anti-HOOK2 antibody (ab154109) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 83 kDa -
Anti-HOOK2 antibody (ab154109) at 1/1000 dilution + mouse liver whole cell lysate at 50 µg
Predicted band size: 83 kDa -
Immunofluorescent analysis of methanol-fixed HeLa cells labeling HOOK2 with ab154109 at 1/200 dilution.
The image in the lower panel was co-stained with Hoechst 33342. -
Immunohistochemical analysis of paraffin embedded MCF7 xenograft tissue labeling HOOK2 with ab154109 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab154109 has been referenced in 3 publications.
- Kumari A et al. Phosphorylation and Pin1 binding to the LIC1 subunit selectively regulate mitotic dynein functions. J Cell Biol 220:N/A (2021). PubMed: 34709360
- Dwivedi D et al. Incorporating Motility in the Motor: Role of the Hook Protein Family in Regulating Dynein Motility. Biochemistry 58:1026-1031 (2019). PubMed: 30702276
- Dwivedi D et al. The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis. J Cell Biol 218:871-894 (2019). PubMed: 30674580