Overview

  • Product name

  • Description

    Rabbit polyclonal to HOOK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 197-380 of Human HOOK2 (UniProt ID: Q96ED9).

  • Positive control

    • WB: HepG2, HCT116 and mouse liver whole cell lysates. IHC-P: Human MCF7 xenograft; Mouse duodenum and testis tissue. ICC/IF: HeLa cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab154109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 83 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance

    HOOK2 is a member of the Hook protein family. Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. HOOK2 localizes to the centrosome through all phases of the cell cycle. Interference with HOOK2 function results in the loss of the radial organization of microtubules suggesting that HOOK2 contributes to the establishment and maintenance of centrosomal structure and function. HOOK2 may contribute to the establishment and maintenance of the pericentrosomal localization of aggresomes by promoting the microtubule-based delivery of protein aggregates to pericentriolar aggresomes.
  • Cellular localization

    Cytoplasmic. Note=Associates with discrete punctate structure that correspond to neither early or late endosomes, lysosomes, multivesicular bodies (MVBs), Golgi complex, endoplasmic reticulum, nor mitochondria.
  • Database links

  • Alternative names

    • A630054I03Rik antibody
    • FLJ26218 antibody
    • h hook2 antibody
    • hHK 2 antibody
    • hHK2 antibody
    • hhook 2 antibody
    • hhook2 antibody
    • HK 2 antibody
    • HK2 antibody
    • HOOK 2 antibody
    • hook homolog 2 (Drosophila) antibody
    • Hook homolog 2 antibody
    • MGC28586 antibody
    • MGC91008 antibody
    see all

Images

  • Paraffin-embedded mouse duodenum tissue stained for HOOK2 using ab154109 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse testis tissue stained for HOOK2 using ab154109 at 1/500 dilution in immunohistochemical analysis.

  • PFA-fixed, Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HOOK2 (green) using ab154109 at 1/500 dilution in ICC/IF.

  • Anti-HOOK2 antibody (ab154109) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size: 83 kDa

  • Anti-HOOK2 antibody (ab154109) at 1/1000 dilution + mouse liver whole cell lysate at 50 µg

    Predicted band size: 83 kDa

  • Immunofluorescent analysis of methanol-fixed HeLa cells labeling HOOK2 with ab154109 at 1/200 dilution.
    The image in the lower panel was co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded MCF7 xenograft tissue labeling HOOK2 with ab154109 at 1/100 dilution.

References

This product has been referenced in:

  • Dwivedi D  et al. Incorporating Motility in the Motor: Role of the Hook Protein Family in Regulating Dynein Motility. Biochemistry 58:1026-1031 (2019). Read more (PubMed: 30702276) »
  • Dwivedi D  et al. The dynein adaptor Hook2 plays essential roles in mitotic progression and cytokinesis. J Cell Biol 218:871-894 (2019). Read more (PubMed: 30674580) »
See all 2 Publications for this product

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