Overview

  • Product name

  • Description

    Rabbit polyclonal to HOOK2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse HOOK2 aa 700 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab101733)

  • Positive control

    • This antibody gave a positive signal in human brain tissue lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab89696 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).

Target

  • Relevance

    HOOK2 is a member of the Hook protein family. Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. HOOK2 localizes to the centrosome through all phases of the cell cycle. Interference with HOOK2 function results in the loss of the radial organization of microtubules suggesting that HOOK2 contributes to the establishment and maintenance of centrosomal structure and function. HOOK2 may contribute to the establishment and maintenance of the pericentrosomal localization of aggresomes by promoting the microtubule-based delivery of protein aggregates to pericentriolar aggresomes.
  • Cellular localization

    Cytoplasmic. Note=Associates with discrete punctate structure that correspond to neither early or late endosomes, lysosomes, multivesicular bodies (MVBs), Golgi complex, endoplasmic reticulum, nor mitochondria.
  • Database links

  • Alternative names

    • A630054I03Rik antibody
    • FLJ26218 antibody
    • h hook2 antibody
    • hHK 2 antibody
    • hHK2 antibody
    • hhook 2 antibody
    • hhook2 antibody
    • HK 2 antibody
    • HK2 antibody
    • HOOK 2 antibody
    • hook homolog 2 (Drosophila) antibody
    • Hook homolog 2 antibody
    • MGC28586 antibody
    • MGC91008 antibody
    see all

Images

  • Anti-HOOK2 antibody (ab89696) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 83 kDa
    Observed band size: 83 kDa
    Additional bands at: 23 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes

References

ab89696 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab89696.
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