Product nameAnti-HOOK2 antibody [EPR8673]
See all HOOK2 primary antibodies
DescriptionRabbit monoclonal [EPR8673] to HOOK2
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Unsuitable for: ICC/IF or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human HOOK2 aa 1-100. The exact sequence is proprietary.
- HeLa, 293T and HepG2 whole cell lysate (ab7900); Human esophageal carcinoma tissue.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (KD)KD = 1.02 x 10 -10 M Learn more about KD
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab133691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).|
|IHC-P||1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
RelevanceHOOK2 is a member of the Hook protein family. Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. HOOK2 localizes to the centrosome through all phases of the cell cycle. Interference with HOOK2 function results in the loss of the radial organization of microtubules suggesting that HOOK2 contributes to the establishment and maintenance of centrosomal structure and function. HOOK2 may contribute to the establishment and maintenance of the pericentrosomal localization of aggresomes by promoting the microtubule-based delivery of protein aggregates to pericentriolar aggresomes.
Cellular localizationCytoplasmic. Note=Associates with discrete punctate structure that correspond to neither early or late endosomes, lysosomes, multivesicular bodies (MVBs), Golgi complex, endoplasmic reticulum, nor mitochondria.
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Immunohistochemical analysis of paraffin-embedded Human esophageal carcinoma tissue labelling HOOK2 with ab133691 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
All lanes : Anti-HOOK2 antibody [EPR8673] (ab133691) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 83 kDa
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD