Anti-HOOK3 antibody (ab173388)
Key features and details
- Mouse polyclonal to HOOK3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HOOK3 antibody
See all HOOK3 primary antibodies -
Description
Mouse polyclonal to HOOK3 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Xenopus laevis -
Immunogen
Recombinant full length protein corresponding to Human HOOK3 aa 1-718. (NP_115786.1).
Sequence:MFSVESLERAELCESLLTWIQTFNVDAPCQTVEDLTNGVVMAQVLQKIDP AYFDENWLNRIKTEVGDNWRLKISNLKKILKGILDYNHEILGQQINDFTL PDVNLIGEHSDAAELGRMLQLILGCAVNCEQKQEYIQAIMMMEESVQHVV MTAIQELMSKESPVSAGNDAYVDLDRQLKKTTEELNEALSAKEEIAQRCH ELDMQVAALQEEKSSLLAENQVLMERLNQSDSIEDPNSPAGRRHLQLQTQ LEQLQEETFRLEAAKDDYRIRCEELEKEISELRQQNDELTTLADEAQSLK DEIDVLRHSSDKVSKLEGQVESYKKKLEDLGDLRRQVKLLEEKNTMYMQN TVSLEEELRKANAARSQLETYKRQVVELQNRLSEESKKADKLDFEYKRLK EKVDSLQKEKDRLRTERDSLKETIEELRCVQAQEGQLTTQGLMPLGSQES SDSLAAEIVTPEIREKLIRLQHENKMLKLNQEGSDNEKIALLQSLLDDAN LRKNELETENRLVNQRLLEVQSQVEELQKSLQDQGSKAEDSVLLKKKLEE HLEKLHEANNELQKKRAIIEDLEPRFNNSSLKIEELQEALRKKEEEMKQM EERYKKYLEKAKSVIRTLDPKQNQGAAPEIQALKNQLQERDRLFHSLEKE YEKTKSQREMEEKYIVSAWYNMGMTLHKKAAEDRLASTGSGQSFLARQRQ ATSSRRSYPGHVQPATAR
Database link: Q86VS8 -
Positive control
- HOOK3 transfected 293T cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab173388 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 83 kDa. |
Target
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Function
Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex. -
Sequence similarities
Belongs to the hook family. -
Cellular localization
Cytoplasm > cytoskeleton. Golgi apparatus. Enriched at the cis-face of the Golgi complex (By similarity). Localizes to microtubule asters in prophase. - Information by UniProt
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Database links
- Entrez Gene: 84376 Human
- Entrez Gene: 320191 Mouse
- Entrez Gene: 443941 Xenopus laevis
- Omim: 607825 Human
- SwissProt: Q86VS8 Human
- SwissProt: Q8BUK6 Mouse
- SwissProt: Q6GQ73 Xenopus laevis
- Unigene: 162852 Human
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Alternative names
- FLJ31058 antibody
- h-hook3 antibody
- hHK3 antibody
see all
Images
Datasheets and documents
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Datasheet download
References (1)
ab173388 has been referenced in 1 publication.
- Villari G et al. Distinct retrograde microtubule motor sets drive early and late endosome transport. EMBO J 39:e103661 (2020). PubMed: 33215754