Key features and details
- Rabbit polyclonal to HOOK3
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HOOK3 antibody
See all HOOK3 primary antibodies
DescriptionRabbit polyclonal to HOOK3
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Xenopus laevis
- WB: HEK-293 whole cell lysate. IHC-P: Human colon cancer tissue. IP: HEK-293 whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity greater than 95%
Our Abpromise guarantee covers the use of ab237529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000.|
|IP||1/200 - 1/2000.|
|IHC-P||1/200 - 1/500.|
FunctionProbably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking (By similarity). Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex.
Sequence similaritiesBelongs to the hook family.
Cellular localizationCytoplasm > cytoskeleton. Golgi apparatus. Enriched at the cis-face of the Golgi complex (By similarity). Localizes to microtubule asters in prophase.
- Information by UniProt
- FLJ31058 antibody
- h-hook3 antibody
- hHK3 antibody
Paraffin-embedded human colon cancer tissue stained for HOOK3 with ab237529 at a 1/300 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Anti-HOOK3 antibody (ab237529) at 1/500 dilution + HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Goat polyclonal to rabbit IgG at 1/50000 dilution
HOOK3 was immunoprecipitated from HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate using 6 µg of ab237529.
Lane 1: Rabbit control IgG in HEK-293 whole cell lysate.
Lane 2: ab237529 (6 µg) in HEK-293 whole cell lysate.
Lane 3: HEK-293 whole cell lysate (20 µg input).
For western blotting an HRP-conjugated Protein G antibody was used as the secondary antibody at a 1/2000 dilution.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab237529 has not yet been referenced specifically in any publications.