Overview

  • Product name

  • Description

    Rabbit polyclonal to HOPX/HOD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HOPX/HOD aa 39-67 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: Q9BPY8

  • Positive control

    • IHC-P: Human kidney tissue. WB: Ramos, A2058 and HEK-293 cell lysate. Flow Cyt: HEK-293 cells.
  • General notes

     This product was previously labelled as HOPX

     

Properties

Applications

Our Abpromise guarantee covers the use of ab230544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 8 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/50.

Target

  • Function

    Atypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy (By similarity). May act as a tumor suppressor.
  • Tissue specificity

    Widely expressed. Expressed in the heart, brain, placenta, lung, skeletal and smooth muscles, uterus, urinary bladder, kidney and spleen. Down-regulated in some types of cancer such as lung cancer, choriocarcinoma, head and neck squamous cell carcinoma and oral squamous cell carcinoma.
  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Form

    There are 2 isoforms produced by alternative splicing.
  • Alternative names

    • CAMEO antibody
    • heart odd homeobox 1 protein antibody
    • HOD antibody
    • Homeodomain-only protein antibody
    • HOP antibody
    • HOP homeobox antibody
    • HOP_HUMAN antibody
    • HOPX antibody
    • LAGY antibody
    • Lung cancer-associated Y protein antibody
    • NECC1 antibody
    • Not expressed in choriocarcinoma clone 1 antibody
    • Not expressed in choriocarcinoma protein 1 antibody
    • OB1 antibody
    • odd homeobox 1 protein antibody
    • Odd homeobox protein 1 antibody
    • OTTHUMP00000158970 antibody
    • SMAP31 antibody
    • TOTO antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human kidney tissue stained for HOPX/HOD with ab230544 at 1/100 dilution in immunohistochemical analysis.

  • All lanes : Anti-HOPX/HOD antibody (ab230544) at 1/1000 dilution

    Lane 1 : Ramos (human Burkitt's lymphoma cell line) cell lysate
    Lane 2 : A2058 cell lysate
    Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 8 kDa

  • Flow Cytometry analysis of HEK-293 (Human epithelial cell line from embryonic kidney) cells, labeling HOPX/HOD with ab230544 at a 1/10 dilution (green) compared to a negative control (blue).

    FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab230544 has not yet been referenced specifically in any publications.

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There are currently no Customer reviews or Questions for ab230544.
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