Key features and details
- Rabbit polyclonal to HOPX/HOD
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HOPX/HOD antibody
See all HOPX/HOD primary antibodies
DescriptionRabbit polyclonal to HOPX/HOD
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Synthetic peptide within Human HOPX/HOD aa 39-67 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q9BPY8
- IHC-P: Human kidney tissue. WB: Ramos, A2058 and HEK-293 cell lysate. Flow Cyt: HEK-293 cells.
This product was previously labelled as HOPX
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab230544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 8 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/50.|
FunctionAtypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy (By similarity). May act as a tumor suppressor.
Tissue specificityWidely expressed. Expressed in the heart, brain, placenta, lung, skeletal and smooth muscles, uterus, urinary bladder, kidney and spleen. Down-regulated in some types of cancer such as lung cancer, choriocarcinoma, head and neck squamous cell carcinoma and oral squamous cell carcinoma.
Sequence similaritiesContains 1 homeobox DNA-binding domain.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing.
- CAMEO antibody
- heart odd homeobox 1 protein antibody
- HOD antibody
Formalin-fixed, paraffin-embedded human kidney tissue stained for HOPX/HOD with ab230544 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-HOPX/HOD antibody (ab230544) at 1/1000 dilution
Lane 1 : Ramos (human Burkitt's lymphoma cell line) cell lysate
Lane 2 : A2058 cell lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 8 kDa
Flow Cytometry analysis of HEK-293 (Human epithelial cell line from embryonic kidney) cells, labeling HOPX/HOD with ab230544 at a 1/10 dilution (green) compared to a negative control (blue).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230544 has been referenced in 1 publication.
- Wang Y et al. Alveolar Differentiation Potency of Human Distal Airway Stem Cells Is Associated with Pulmonary Pathological Conditions. Stem Cells Int 2019:7123078 (2019). PubMed: 31281383