Anti-HOPX/HOD antibody (ab230544)
Key features and details
- Rabbit polyclonal to HOPX/HOD
- Suitable for: Flow Cyt (Intra), WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HOPX/HOD antibody
See all HOPX/HOD primary antibodies -
Description
Rabbit polyclonal to HOPX/HOD -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human HOPX/HOD aa 39-67 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
Database link: Q9BPY8 -
Positive control
- IHC-P: Human kidney tissue. WB: Ramos, A2058 and HEK-293 cell lysate. Flow Cyt (Intra): HEK-293 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/50.
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WB |
1/1000. Predicted molecular weight: 8 kDa.
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IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/10 - 1/50. |
WB
1/1000. Predicted molecular weight: 8 kDa. |
IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Atypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy (By similarity). May act as a tumor suppressor. -
Tissue specificity
Widely expressed. Expressed in the heart, brain, placenta, lung, skeletal and smooth muscles, uterus, urinary bladder, kidney and spleen. Down-regulated in some types of cancer such as lung cancer, choriocarcinoma, head and neck squamous cell carcinoma and oral squamous cell carcinoma. -
Sequence similarities
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 84525 Human
- Omim: 607275 Human
- SwissProt: Q9BPY8 Human
- Unigene: 619396 Human
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Form
There are 2 isoforms produced by alternative splicing. -
Alternative names
- CAMEO antibody
- heart odd homeobox 1 protein antibody
- HOD antibody
see all
Images
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Formalin-fixed, paraffin-embedded human kidney tissue stained for HOPX/HOD with ab230544 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-HOPX/HOD antibody (ab230544) at 1/1000 dilution
Lane 1 : Ramos (human Burkitt's lymphoma cell line) cell lysate
Lane 2 : A2058 cell lysate
Lane 3 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lysates/proteins at 35 µg per lane.
Developed using the ECL technique.
Predicted band size: 8 kDa -
Intracellular Flow Cytometry analysis of HEK-293 (Human epithelial cell line from embryonic kidney) cells, labeling HOPX/HOD with ab230544 at a 1/10 dilution (green) compared to a negative control (blue).
FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (4)
ab230544 has been referenced in 4 publications.
- Papes F et al. Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content. Nat Commun 13:2387 (2022). PubMed: 35501322
- Wang X et al. Intrapulmonary distal airway stem cell transplantation repairs lung injury in chronic obstructive pulmonary disease. Cell Prolif 54:e13046 (2021). PubMed: 33960563
- Zhou Y et al. Stable Long-Term Culture of Human Distal Airway Stem Cells for Transplantation. Stem Cells Int 2021:9974635 (2021). PubMed: 34567131
- Wang Y et al. Alveolar Differentiation Potency of Human Distal Airway Stem Cells Is Associated with Pulmonary Pathological Conditions. Stem Cells Int 2019:7123078 (2019). PubMed: 31281383