Product nameAnti-HOPX/HOD antibody [EPR18760]
See all HOPX/HOD primary antibodies
DescriptionRabbit monoclonal [EPR18760] to HOPX/HOD
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Mouse, Rat
Recombinant full length protein corresponding to Mouse HOPX/HOD aa 1 to the C-terminus.
Database link: Q8R1H0
- WB: Mouse lung and heart lysates; Rat lung, kidney, placenta and heart lysates. IP: Mouse lung lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab195974 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 8 kDa).|
FunctionAtypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy (By similarity). May act as a tumor suppressor.
Tissue specificityWidely expressed. Expressed in the heart, brain, placenta, lung, skeletal and smooth muscles, uterus, urinary bladder, kidney and spleen. Down-regulated in some types of cancer such as lung cancer, choriocarcinoma, head and neck squamous cell carcinoma and oral squamous cell carcinoma.
Sequence similaritiesContains 1 homeobox DNA-binding domain.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing.
- CAMEO antibody
- heart odd homeobox 1 protein antibody
- HOD antibody
Lanes 1-4 : Anti-HOPX/HOD antibody [EPR18760] (ab195974) at 1/2000 dilution
Lanes 5-6 : Anti-HOPX/HOD antibody [EPR18760] (ab195974) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Mouse heart lysate
Lane 3 : Rat lung lysate
Lane 4 : Rat kidney lysate
Lane 5 : Rat placenta lysate
Lane 6 : Rat heart lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 8 kDa
Observed band size: 13 kDa why is the actual band size different from the predicted?
Blocking and Diluting buffer and concentration: 5% NFDM/TBST.
Exposure times: Lane 1/2: 10 seconds; Lane 3/4/5: 3 minutes; Lane 6: 15 seconds.
HOPX/HOD was immunoprecipitated from 0.35 mg of Mouse lung lysate with ab195974 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab195974 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse lung lysate 10 μg (Input).
Lane 2: ab195974 IP in Mouse lung lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195974 in Mouse lung lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.
ab195974 has not yet been referenced specifically in any publications.