Overview

  • Product name

    Anti-HOPX/HOD antibody [EPR18760]
    See all HOPX/HOD primary antibodies
  • Description

    Rabbit monoclonal [EPR18760] to HOPX/HOD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein corresponding to Mouse HOPX/HOD aa 1 to the C-terminus.
    Database link: Q8R1H0

  • Positive control

    • WB: Mouse lung and heart lysates; Rat lung, kidney, placenta and heart lysates. IP: Mouse lung lysate.
  • General notes

     This product was previously labelled as HOPX

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab195974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/40.
WB 1/1000. Detects a band of approximately 13 kDa (predicted molecular weight: 8 kDa).

Target

  • Function

    Atypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy (By similarity). May act as a tumor suppressor.
  • Tissue specificity

    Widely expressed. Expressed in the heart, brain, placenta, lung, skeletal and smooth muscles, uterus, urinary bladder, kidney and spleen. Down-regulated in some types of cancer such as lung cancer, choriocarcinoma, head and neck squamous cell carcinoma and oral squamous cell carcinoma.
  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Form

    There are 2 isoforms produced by alternative splicing.
  • Alternative names

    • CAMEO antibody
    • heart odd homeobox 1 protein antibody
    • HOD antibody
    • Homeodomain-only protein antibody
    • HOP antibody
    • HOP homeobox antibody
    • HOP_HUMAN antibody
    • HOPX antibody
    • LAGY antibody
    • Lung cancer-associated Y protein antibody
    • NECC1 antibody
    • Not expressed in choriocarcinoma clone 1 antibody
    • Not expressed in choriocarcinoma protein 1 antibody
    • OB1 antibody
    • odd homeobox 1 protein antibody
    • Odd homeobox protein 1 antibody
    • OTTHUMP00000158970 antibody
    • SMAP31 antibody
    • TOTO antibody
    see all

Images

  • Lanes 1-4 : Anti-HOPX/HOD antibody [EPR18760] (ab195974) at 1/2000 dilution
    Lanes 5-6 : Anti-HOPX/HOD antibody [EPR18760] (ab195974) at 1/1000 dilution

    Lane 1 : Mouse lung lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Rat lung lysate
    Lane 4 : Rat kidney lysate
    Lane 5 : Rat placenta lysate
    Lane 6 : Rat heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 8 kDa
    Observed band size: 13 kDa
    why is the actual band size different from the predicted?



    Blocking and Diluting buffer and concentration: 5% NFDM/TBST.

    Exposure times: Lane 1/2: 10 seconds; Lane 3/4/5: 3 minutes; Lane 6: 15 seconds.

     

  • HOPX/HOD was immunoprecipitated from 0.35 mg of Mouse lung lysate with ab195974 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab195974 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse lung lysate 10 μg (Input).

    Lane 2: ab195974 IP in Mouse lung lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195974 in Mouse lung lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

References

ab195974 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Permeabilization
No
Specification
heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Sep 26 2018

Application
Western blot
Sample
Mouse Tissue lysate - whole (lung, NIH-3T3)
Gel Running Conditions
Non-reduced Denaturing (15%)
Loading amount
50 µg
Specification
lung, NIH-3T3
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Zheng Wang

Verified customer

Submitted Mar 13 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up