Recombinant
RabMAb

Recombinant Anti-HOPX/HOD antibody [EPR18760] - BSA and Azide free (ab223537)

Overview

  • Product name

    Anti-HOPX/HOD antibody [EPR18760] - BSA and Azide free
    See all HOPX/HOD primary antibodies
  • Description

    Rabbit monoclonal [EPR18760] to HOPX/HOD - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein aa 1 to the C-terminus.
    Database link: Q8R1H0

  • Positive control

    • WB: Mouse lung and heart lysates; Rat lung, kidney, placenta and heart lysates. IP: Mouse lung lysate.
  • General notes

    ab223537 is the carrier-free version of ab195974 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab223537 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as HOPX

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab223537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 13 kDa (predicted molecular weight: 8 kDa).

Target

  • Function

    Atypical homeodomain protein which does not bind DNA and is required to modulate cardiac growth and development. Acts via its interaction with SRF, thereby modulating the expression of SRF-dependent cardiac-specific genes and cardiac development. Prevents SRF-dependent transcription either by inhibiting SRF binding to DNA or by recruiting histone deacetylase (HDAC) proteins that prevent transcription by SRF. Overexpression causes cardiac hypertrophy (By similarity). May act as a tumor suppressor.
  • Tissue specificity

    Widely expressed. Expressed in the heart, brain, placenta, lung, skeletal and smooth muscles, uterus, urinary bladder, kidney and spleen. Down-regulated in some types of cancer such as lung cancer, choriocarcinoma, head and neck squamous cell carcinoma and oral squamous cell carcinoma.
  • Sequence similarities

    Contains 1 homeobox DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Form

    There are 2 isoforms produced by alternative splicing.
  • Alternative names

    • CAMEO antibody
    • heart odd homeobox 1 protein antibody
    • HOD antibody
    • Homeodomain-only protein antibody
    • HOP antibody
    • HOP homeobox antibody
    • HOP_HUMAN antibody
    • HOPX antibody
    • LAGY antibody
    • Lung cancer-associated Y protein antibody
    • NECC1 antibody
    • Not expressed in choriocarcinoma clone 1 antibody
    • Not expressed in choriocarcinoma protein 1 antibody
    • OB1 antibody
    • odd homeobox 1 protein antibody
    • Odd homeobox protein 1 antibody
    • OTTHUMP00000158970 antibody
    • SMAP31 antibody
    • TOTO antibody
    see all

Images

  • This IP data was generated using the same anti-HOPX/HOD antibody clone [EPR18760] in a different buffer formulation (cat# ab195974).

    HOPX/HOD was immunoprecipitated from 0.35 mg of Mouse lung lysate with ab195974 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab195974 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse lung lysate 10 μg (Input).

    Lane 2: ab195974 IP in Mouse lung lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab195974 in Mouse lung lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

References

ab223537 has not yet been referenced specifically in any publications.

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