Product nameHORAC Assay Kit
See all Oxidative Stress kits
HORAC Assay Kit (ab242299) is a fast and reliable kit for the direct measurement of HORAC antioxidant capacity.
Each kit provides sufficient reagents to perform up to 192 assays, including blanks, antioxidant standards and unknown samples. The assay is designed for use in single plate microplate readers as well as readers with high-throughput capabilities.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 192 tests 48 tests 96-well Microtiter Plate 2 units 2 units Assay Diluent (2X) 1 x 50ml 1 x 10ml Fenton Reagent 1 x 5ml 1 x 1ml Gallic Acid Antioxidant Standard 1 x 1.5ml 1 x 1.5ml HORAC Fluorescein Probe (100X) 1 x 0.5ml 1 x 75µl Hydroxyl Radical Initiator (5X) 1 x 1ml 1 x 200µl
The figure demonstrates typical Assay results. One should use the data for reference only. This data should not be used to interpret or calculate actual sample results.
Calculate the area under the curve (AUC) for each sample and standard using the final assay values and the linear regression formula as shown in the protocol booklet.
Calculate the Net AUC by subtracting the Blank AUC from the AUC of each sample and standard.
ab242299 has not yet been referenced specifically in any publications.