Overview

  • Product name

    Anti-Hormone sensitive lipase/HSL antibody
    See all Hormone sensitive lipase/HSL primary antibodies
  • Description

    Rabbit polyclonal to Hormone sensitive lipase/HSL
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human Hormone sensitive lipase/HSL (C terminal).

  • Positive control

    • Human lymph node tissue lysate.
  • General notes

     This product was previously labelled as Hormone sensitive lipase

     

Applications

Our Abpromise guarantee covers the use of ab45422 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2.5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 117 kDa).

Target

Images

  • ab45422 staining Hormone sensitive lipase/HSL in the HeLa cell line from Human cervical cancer by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/200 in PBS) for 16 hour at 22°C. ab150081 an Alexa Fluor®488-conjugated Goat anti-rabbit IgG H&L (1/200) polyclonal was used as the secondary antibody. Nuclei were stained with DAPI.MeOH-fixed samples were also evaluated and failed to produce the expected staining pattern.

    See Abreview

  • Lane 1 : Anti-Hormone sensitive lipase/HSL antibody (ab45422) at 0.5 µg/ml
    Lane 2 : Anti-Hormone sensitive lipase/HSL antibody (ab45422) at 1 µg/ml

    All lanes : Human lymph node tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 117 kDa
    Observed band size: 90 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 38 kDa, 86 kDa, 88 kDa. We are unsure as to the identity of these extra bands.

  • Immunofluorescence of Lipe in Human Lymph Node cells using ab45422 at 20 ug/ml.

References

This product has been referenced in:

  • Cunarro J  et al. p107 Deficiency Increases Energy Expenditure by Inducing Brown-Fat Thermogenesis and Browning of White Adipose Tissue. Mol Nutr Food Res 63:e1801096 (2019). Read more (PubMed: 30383332) »
  • Lopes MA  et al. LLC tumor cells-derivated factors reduces adipogenesis in co-culture system. Heliyon 4:e00708 (2018). Read more (PubMed: 30094378) »
See all 21 Publications for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Specification
HeLa
Fixative
Paraformaldehyde

Dr. Kirk Mcmanus

Verified customer

Submitted Nov 05 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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