Recombinant Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330] (ab76492)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2330] to Hormone sensitive lipase/HSL (phospho S855)
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330]
See all Hormone sensitive lipase/HSL primary antibodies -
Description
Rabbit monoclonal [EPR2330] to Hormone sensitive lipase/HSL (phospho S855) -
Host species
Rabbit -
Specificity
Detects HSL/LIPE only when phosphorylated on Serine 855 -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human skeletal muscle lysate
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2330 -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76492 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 117 , 83 kDa.
Predicted molecular weight: 117 kDa (isoform 1) or 83 kDa (isoform 2). |
Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 117 , 83 kDa. Predicted molecular weight: 117 kDa (isoform 1) or 83 kDa (isoform 2). |
Target
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Function
In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production. -
Pathway
Glycerolipid metabolism; triacylglycerol degradation. -
Sequence similarities
Belongs to the 'GDXG' lipolytic enzyme family. -
Cellular localization
Cell membrane. Membrane > caveola. Cytoplasm > cytosol. Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation. - Information by UniProt
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Database links
- Entrez Gene: 3991 Human
- Entrez Gene: 16890 Mouse
- Omim: 151750 Human
- SwissProt: Q05469 Human
- SwissProt: Q6NSL7 Human
- SwissProt: P54310 Mouse
- Unigene: 656980 Human
- Unigene: 158548 Mouse
see all -
Alternative names
- Hormone sensitive lipase antibody
- Hormone sensitive lipase testicular isoform antibody
- Hormone-sensitive lipase antibody
see all
Images
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All lanes : Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330] (ab76492) at 1/5000 dilution
Lane 1 : Human skeletal muscle lysate, untreated
Lane 2 : Human skeletal muscle lysate, treated with LP
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/1000 dilution
Predicted band size: 117 , 83 kDa
Observed band size: 83 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab76492 has been referenced in 1 publication.
- Chadwick ML et al. Combined mTOR and MEK inhibition is an effective therapy in a novel mouse model for angiosarcoma. Oncotarget 9:24750-24765 (2018). PubMed: 29872503