Recombinant
RabMAb

Recombinant Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330] (ab76492)

Overview

  • Product name

    Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330]
    See all Hormone sensitive lipase/HSL primary antibodies
  • Description

    Rabbit monoclonal [EPR2330] to Hormone sensitive lipase/HSL (phospho S855)
  • Host species

    Rabbit
  • Specificity

    Detects HSL/LIPE only when phosphorylated on Serine 855
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human Hormone sensitive lipase/HSL.

  • Positive control

    • Human skeletal muscle lysate
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

     

     This product was previously labelled as Hormone sensitive lipase

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab76492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/1000 - 1/5000. Predicted molecular weight: 117 kDa (isoform 1) or 83 kDa (isoform 2).

    Is unsuitable for ICC/IF, IHC-P or IP.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      In adipose tissue and heart, it primarily hydrolyzes stored triglycerides to free fatty acids, while in steroidogenic tissues, it principally converts cholesteryl esters to free cholesterol for steroid hormone production.
    • Pathway

      Glycerolipid metabolism; triacylglycerol degradation.
    • Sequence similarities

      Belongs to the 'GDXG' lipolytic enzyme family.
    • Cellular localization

      Cell membrane. Membrane > caveola. Cytoplasm > cytosol. Found in the high-density caveolae. Translocates to the cytoplasm from the caveolae upon insulin stimulation.
    • Information by UniProt
    • Database links

    • Alternative names

      • Hormone sensitive lipase antibody
      • Hormone sensitive lipase testicular isoform antibody
      • Hormone-sensitive lipase antibody
      • HSL antibody
      • LHS antibody
      • Lipase hormone sensitive antibody
      • LIPE antibody
      • LIPS_HUMAN antibody
      see all

    Images

    • All lanes : Anti-Hormone sensitive lipase/HSL (phospho S855) antibody [EPR2330] (ab76492) at 1/5000 dilution

      Lane 1 : Human
      skeletal muscle lysate, untreated
      Lane 2 : Human
      skeletal muscle lysate, treated with LP

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labeled goat anti-rabbit at 1/1000 dilution

      Predicted band size: 117 , 83 kDa
      Observed band size: 83 kDa
      why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    • Chadwick ML  et al. Combined mTOR and MEK inhibition is an effective therapy in a novel mouse model for angiosarcoma. Oncotarget 9:24750-24765 (2018). Read more (PubMed: 29872503) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Answer

    Thank you for your inquiry.

    The Serine 855 of human HSL is equivalent to Serine 559 of mouse HSL, and this would recognize the Serine 559 of mouse HSL. The orthologous residue for isoform 2 of the rat HSL would be Serine 565, and this antibody should recognize it as well, although the rat is not recommended based on our WB analysis.


    I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up