Key features and details
- Sensitivity: 2.217 ng/ml
- Range: 6.25 ng/ml - 400 ng/ml
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Horse
Product nameHorse IgG ELISA Kit
See all IgG kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 10%
Sample typeSerum, Plasma
Assay typeSandwich (quantitative)
Range6.25 ng/ml - 400 ng/ml
Sample specific recovery Sample type Average % Range Serum > 85 % - %
Assay durationMultiple steps standard assay
Species reactivityReacts with: Horse
Abcam’s IgG Horse ELISA kit (ab190531) is an in vitro enzyme-linked immunosorbent assay (ELISA) for the quantitative measurement of IgG in horse serum and plasma.
In this assay the IgG present in samples reacts with the anti-IgG antibodies which have been adsorbed to the surface of polystyrene microtitre wells. After the removal of unbound proteins by washing, anti-IgG antibodies conjugated with horseradish peroxidase (HRP), are added. These enzyme-labeled antibodies form complexes with the previously bound IgG. Following another washing step, the enzyme bound to the immunosorbent is assayed by the addition of a chromogenic substrate, 3,3’,5,5’-tetramethylbenzidine (TMB). The quantity of bound enzyme varies directly with the concentration of IgG in the sample tested; thus, the absorbance, at 450 nm, is a measure of the concentration of IgG in the test sample. The quantity of IgG in the test sample can be interpolated from the standard curve constructed from the standards, and corrected for sample dilution.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 50ml 5X Diluent Concentrate 1 x 50ml Chromogen Substrate Solution 1 x 12ml IgG Horse Antibody coated microwells 1 unit IgG Horse Calibrator 1 vial IgG Horse HRP Conjugate 1 vial Stop Solution 1 x 12ml
- Ig gamma 1 chain C region
- Ig gamma 2 chain C region
- Ig gamma 3 chain C region
ab190531 has not yet been referenced specifically in any publications.