Key features and details
- Sensitivity: 49 pg/ml
- Range: 49.15 pg/ml - 12000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Horse, Human
Product nameHorse MMP9 ELISA kit
See all MMP9 kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range49.15 pg/ml - 12000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 118.1 95% - 147% Serum 112.5 92% - 129% Plasma 100.4 84% - 116%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Horse, Human
Horse MMP-9 ELISA Kit is designed for the quantitative determination of Horse MMP-9 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for MMP-9 coated on a 96-well plate. Standards and samples are pipetted into the wells and MMP-9 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Horse MMP-9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP-9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Detects horse and human MMP9.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Horse MMP9 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Horse MMP9 Detection Antibody 2 vials Horse MMP9 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml
FunctionMay play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-
-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Tissue specificityProduced by normal alveolar macrophages and granulocytes.
Involvement in diseaseIntervertebral disc disease
Metaphyseal anadysplasia 2
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 3 fibronectin type-II domains.
Contains 4 hemopexin repeats.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsProcessing of the precursor yields different active forms of 64, 67 and 82 kDa. Sequentially processing by MMP3 yields the 82 kDa matrix metalloproteinase-9.
N- and O-glycosylated.
Cellular localizationSecreted, extracellular space, extracellular matrix.
- Information by UniProt
- 82 kDa matrix metalloproteinase-9
- 92 kDa gelatinase
- 92 kDa type IV collagenase
ab272029 has not yet been referenced specifically in any publications.