Key features and details
- Sensitivity: 8 pg/ml
- Range: 8.19 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Horse, Human
Product nameHorse PDGF-BB ELISA kit
See all PDGF B kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range8.19 pg/ml - 2000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 104.7 93% - 113% Serum 118.6 107% - 136% Plasma 104.8 96% - 117%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Horse, Human
Horse PDGF-BB ELISA Kit is designed for the quantitative determination of Horse PDGF-BB in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for PDGF-BB coated on a 96-well plate. Standards and samples are pipetted into the wells and PDGF-BB present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Horse PDGF-BB antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of PDGF-BB bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Detects horse and human PDGF-BB.
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent 1 x 15ml 800X HRP-Streptavidin Concentrate 1 x 200µl Anti-Horse PDGF-BB coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Horse PDGF-BB Detection Antibody 2 vials Horse PDGF-BB Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml
- Information by UniProt
- c sis
ab272032 has not yet been referenced specifically in any publications.