Product nameHorse Resistin ELISA kit
See all Resistin kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range46.88 pg/ml - 3000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 90.68 71% - 106% Serum 120.9 113% - 132% Plasma 113.6 101% - 134%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Horse, Human
Horse Resistin ELISA Kit is designed for the quantitative determination of Horse Resistin in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for Resistin coated on a 96-well plate. Standards and samples are pipetted into the wells and Resistin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Horse Resistin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Resistin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Detects horse and human Resistin.
Tested applicationsSuitable for: Sandwich ELISAmore details
PlatformMicroplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 100X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Anti-Horse Resistin coated Microplate (12 x 8 wells) 1 unit Assay Diluent C 1 x 30ml Biotinylated Anti-Horse Resistin Detection Antibody 2 vials Horse Resistin Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Energy Metabolism
FunctionHormone that seems to suppress insulin ability to stimulate glucose uptake into adipose cells. Potentially links obesity to diabetes.
Tissue specificityExpressed only in fatty tissues.
Sequence similaritiesBelongs to the resistin/FIZZ family.
- Information by UniProt
- Adipose tissue specific secretory factor
- Adipose tissue-specific secretory factor
Our Abpromise guarantee covers the use of ab272031 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab272031 has not yet been referenced specifically in any publications.