Key features and details
- Sensitivity: 4 pg/ml
- Range: 16.38 pg/ml - 4000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Horse, Human
Product nameHorse TIMP2 ELISA kit
See all TIMP2 kits
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12%
Sample typeCell culture supernatant, Serum, Plasma
Assay typeSandwich (quantitative)
Range16.38 pg/ml - 4000 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 141.3 131% - 149% Serum 141.2 135% - 148% Plasma 143.3 137% - 149%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Horse, Human
Horse TIMP-2 ELISA Kit is designed for the quantitative determination of Horse TIMP-2 in cell culture supernatants, plasma and serum samples.
This assay employs an antibody specific for TIMP-2 coated on a 96-well plate. Standards and samples are pipetted into the wells and TIMP-2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Horse TIMP-2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TIMP-2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Detects horse and human TIMP2.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Horse TIMP2 coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Horse TIMP2 Detection Antibody 2 vials Horse TIMP2 Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml
FunctionComplexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19.
Sequence similaritiesBelongs to the protease inhibitor I35 (TIMP) family.
Contains 1 NTR domain.
modificationsThe activity of TIMP2 is dependent on the presence of disulfide bonds.
- Information by UniProt
- CSC 21K
ab272033 has not yet been referenced specifically in any publications.