Key features and details
- Rabbit polyclonal to Host cell factor C1 regulator 1
- Suitable for: ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Host cell factor C1 regulator 1 antibody
DescriptionRabbit polyclonal to Host cell factor C1 regulator 1
Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human cerebellum tissue. ICC/IF: A549 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab254955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionRegulates HCFC1 activity by modulating its subcellular localization. Overexpression of HCFC1R1 leads to accumulation of HCFC1 in the cytoplasm. HCFC1R1-mediated export may provide the pool of cytoplasmic HCFC1 required for import of virion-derived VP16 into the nucleus.
Tissue specificityWidely expressed.
Cellular localizationCytoplasm. Nucleus. Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner.
- Information by UniProt
- HCF 1 beta propeller interacting protein antibody
- HCF-1 beta-propeller-interacting protein antibody
- HCF1 beta propeller interacting protein antibody
Paraffin-embedded human cerebellum tissue stained for Host cell factor C1 regulator 1 using ab254955 at 1/20 dilution in immunohistochemical analysis.
PFA fixed, Triton X-100 permeabilized A549 (Human lung carcinoma cell line) cells labeling Host cell factor C1 regulator 1 using ab254955 at 4µg/ml (green) in ICC/IF.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254955 has not yet been referenced specifically in any publications.