Key features and details
- Rabbit polyclonal to HOXA1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-HOXA1 antibody
See all HOXA1 primary antibodies
DescriptionRabbit polyclonal to HOXA1
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Zebrafish
Synthetic peptide corresponding to Human HOXA1 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: P49639
- IHC-P: Human colon tissue. WB: HEK-293T and HeLa whole cell lysate (ab150035). ICC/IF: HeLa cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230513 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 36 kDa.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/500.|
FunctionSequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Acts on the anterior body structures. Seems to act in the maintenance and/or generation of hindbrain segments.
Involvement in diseaseAthabaskan brainstem dysgenesis syndrome
Sequence similaritiesBelongs to the Antp homeobox family. Labial subfamily.
Contains 1 homeobox DNA-binding domain.
- Information by UniProt
- BSAS antibody
- Homeo box A1 antibody
- Homeobox 1F antibody
All lanes : Anti-HOXA1 antibody (ab230513) at 1/500 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Predicted band size: 36 kDa
Formalin-fixed, paraffin-embedded human colon tissue stained for HOXA1 using ab230513 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for HOXA1 (red) using ab230513 at 1/100 dilution in ICC/IF, followed by DyLight® 594-conjugated secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230513 has been referenced in 3 publications.
- Liu J et al. HOXA1 upregulation is associated with poor prognosis and tumor progression in breast cancer. Exp Ther Med 17:1896-1902 (2019). PubMed: 30783466
- Mao Y et al. circEIF4G2 modulates the malignant features of cervical cancer via the miR-218/HOXA1 pathway. Mol Med Rep 19:3714-3722 (2019). PubMed: 30896864
- Tao C et al. miRNA-99a inhibits cell invasion and migration in liver cancer by directly targeting HOXA1. Oncol Lett 17:5108-5114 (2019). PubMed: 31186723